| Neuro-protective effects of CNTF on hippocampal neurons via an unknown signal transduction pathway |
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| 作者姓名： | LI Ping WANG Zongwen YAN Jin Li Zhaoshen JIANG Chunlei NI Xin YANG Yongji LIU Fang LU Changlin |
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| 作者单位： | [1]Department of Psychology, Second Military Medical University, Shanghai 200433, China [2]Changhai Hospital, Second Military Medical University, Shanghai 200433, China [3]Laboratory of Stress Medicine, Second Military Medical University, Shanghai 200433, China [4]School of Basic Medicine, Second Military Medical University, Shanghai 200433,China |
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| 基金项目： | Acknowledgements We thank Professor He C. from the Department of Neurophysiology of the Second Military Medical University for providing us CNTF reagents and Dr. Norman Fichtenberg from Wayne State University for editing suggestions. This work was supported by the National Natural Science Foundation of China （Grant Nos. 30270364 ＆ 30572080）, Medicine and Pharmacy Foundation of PLA （Grant No. 04M021 and ＂11.5＂ Special Fund） and Foundation of Second Military Medical University （Grant No. 05JS02）. |
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| 摘 要： | Stress is one of the leading contributing factors for psychosomatic diseases of modern society. Prolonged or strong stress may cause more release of glutamic acid (Glu) transmitter from hippocampal neurons. As most hippocampal neurons are glutaminergic ne…
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| 关 键 词： | CNTF L-NMDA 海马神经元 JAK/STATs PTPi-2 NMDA |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005-06-202005-11-23 |
Neuro-protective effects of CNTF on hippocampal neurons via an unknown signal transduction pathway |
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| LI Ping WANG Zongwen YAN Jin Li Zhaoshen JIANG Chunlei NI Xin YANG Yongji LIU Fang LU Changlin.Neuro-protective effects of CNTF on hippocampal neurons via an unknown signal transduction pathway[J].Chinese Science Bulletin,2006,51(1):48-53. |
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| Authors: | Ping Li Zongwen Wang Jin Yan Zhaoshen Li Chunlei Jiang Xin Ni Yongji Yang Fang Liu Changlin Lu |
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| Institution: | (1) Department of Psychology, Second Military Medical University, Shanghai, 200433, China;(2) Changhai Hospital, Second Military Medical University, Shanghai, 200433, China;(3) Laboratory of Stress Medicine, Second Military Medical University, Shanghai, 200433, China;(4) School of Basic Medicine, Second Military Medical University, Shanghai, 200433, China |
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| Abstract: | In our previous study, we proposed that there may be an unknown pathway in the upper stream of the known signal transduction pathway of Ciliary neurotrophic factor (CNTF) that mediates the neuro-protective function of CNTF. In the present experiment, we observed that the neuro-protective function of the non-classic signal transduction path- way in a L-NMDA (a glutamic acid ion type receptor atagonist) induced hippocampal neuron injury model, using primary culture rat hippocampal neurons, con- tinuous photography and gp130 immunohistochemi- cal assay. The results showed that L-NMDA induced injurious reaction of hippocampal neurons, and CNTF was able to inhibit the toxic action of L-NMDA on hippocampal neurons. Additionally, when JAK/STATs in the known classic signal transduction pathway of CNTF were blocked by PTPi-2, the protective effect of CNTF against L-NMDA injury still existed. L-NMDA caused a rapid increase in the concentration of hip- pocampal intracellular free Ca2 ]i. CNTF was able to attenuate L-NMDA-induced elevation of Ca2 ]i, and blocking JAK/STATs in the known classic signal trans- duction pathway of CNTF did not affect L-NMDA- induced elevation of Ca2 ]i, indicating that, apart from the known classic signal transduction pathway, there may be some other transduction pathways for CNTF to exert the protective effect on hippocampal neurons, and this pathway is related to Ca2 ]. |
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| Keywords: | CNTF L-NMDA hippocampal neurons JAK/STATs PTPi-2 NMDA receptor [Ca2 ] |
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