| Reovirus, isolated from SARS patients |
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| 作者姓名： | DUANQing ZHUHong YANGYi LIWeihua ZHOUYusen HEJun HEKun ZHANGHaojie ZHOUTao SONGLihua GANYonghua TANHua JINBaofeng LIHuiyan ZUOTingting CHENDehui ZHANGXuemin |
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| 作者单位： | [1]InstituteofMicrobiologyandEpidemiology,AcademyofMilitaryMedicalSciences,Beijing100071,China [2]ationalCenterofBiomedicalAnalysis,Beijing100850,China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Beijing has been severely affected by SARS, and SARS-associated coronavirus has been confirmed as its cause. However, clinical and experimental evidence implicates the possibility of co-infection. In this report, reovirus was isolated from throat swabs of SARS patients, including the first case in Beijing and her mother. Identification with the electron microscopy revealed the characteristic features of reovirus. 24 of 38 samples from other SARS cases were found to have serologic responses to the reovirus. Primers designed for reovirus have amplified several fragments of DNA, one of which was sequenced (S2 gene fragment), which indicates it as a unique reovirus (orthoreovirus). Preliminary animal experiment showed that inoculation of the reovirus in mice caused death with atypical pneumonia. Nevertheless, the association of reovirus with SARS outbreak requires to be further investigated.
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| 关 键 词： | 呼吸道肠道病毒 非典型肺炎 SARS 传染病防治 电子显微镜 |
| 收稿时间： | 2003-06-10 |
| 修稿时间： | 2003-06-12 |
Reovirus, isolated from SARS patients |
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| DUANQing ZHUHong YANGYi LIWeihua ZHOUYusen HEJun HEKun ZHANGHaojie ZHOUTao SONGLihua GANYonghua TANHua JINBaofeng LIHuiyan ZUOTingting CHENDehui ZHANGXuemin.Reovirus, isolated from SARS patients[J].Chinese Science Bulletin,2003,48(13):1293-1296. |
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| Authors: | Qing Duan Hong Zhu Yi Yang Weihua Li Yusen Zhou Jun He Kun He Haojie Zhang Tao Zhou Lihua Song Yonghua Gan Hua Tan Baofeng Jin Huiyan Li Tingting Zuo Dehui Chen Xuemin Zhang |
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| Institution: | DUAN Qing1, ZHU Hong1, YANG Yi2, LI Weihua2, ZHOU Yusen1, HE Jun1, HE Kun2, ZHANG Haojie1, ZHOU Tao2, SONG Lihua1, GAN Yonghua1, TAN Hua1, JIN Baofeng2, LI Huiyan2, ZUO Tingting1, CHEN Dehui2 & ZHANG Xuemin2 1. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China; 2. National Center of Biomedical Analysis, Beijing 100850, China |
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| Abstract: | Beijing has been severely affected by SARS, and SARS-associated coronavirus has been confirmed as its cause. However, clinical and experimental evidence implicates the possibility of co-infection. In this report, reovirus was isolated from throat swabs of SARS patients, including the first case in Beijing and her mother. Identification with the electron microscopy revealed the characteristic features of reovirus. 24 of 38 samples from other SARS cases were found to have serologic responses to the reovirus. Primers designed for reovirus have amplified several fragments of DNA, one of which was sequenced (S2 gene fragment), which indicates it as a unique reovirus (orthoreovirus). Preliminary animal experiment showed that inoculation of the reovirus in mice caused death with atypical pneumonia. Nevertheless, the association of reovirus with SARS outbreak requires to be further investigated. |
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| Keywords: | reovirus coronavirus SARS electron microscopy |
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