Molecular tagging of a genic male-sterile gene in rice |
| |
Authors: | Xuewei Chen Shigui Li Wenming Wang Hanyun Li Kaida Zhou Lihuang Zhu |
| |
Institution: | (1)Institute of Genetics, Chinese Academy of Science, Beijing 100101 ,China;
(2)Rice Research Institute, Sichuan Agricultural University, Chengdu 611130 ,China |
| |
Abstract: | The sterility of Pingxiang male-sterile rice (Pms), possibly derided from a spontaneous mutation in Pingxiang fertile rice (Pmf), was previously reported to be controlled by a single dominant nuclear gene. It can be restored to fertility either by a dominant epistatic gene or by higher temperature treatment at the early stage of inflorescence development. In order to tag the genic male-sterile gene, Pms, Pmf and Ce 64, a cytoplasmic male-sterile restoring line without the epistatic gene for Pms, were used to construct mapping populations. Two segregation populations, “(Pms/Ce 64) F1s (sterile plant)//Pmf ” F1 and “Pms//(Pmf/Ce 64) F1” F1, were simultaneously developed. Subsequently, the genic male- sterile gene was mapped between a simple sequence length polymorphism marker, RM228, and a restriction fragment length polymorphism marker, G2155, with distances of 14.9 and 2.6 cM, respectively. The tagged dominant genic male-sterile gene is temporarily designated Ms-p. |
| |
Keywords: | rice genie male-sterility molecular mapping molecular marker gene localization |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|