| Screening of HLA-A2 restricted CTL epitopes using molecular simulation |
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| 作者姓名： | GENG Miao WU Yuzhang LIN Zhihua JIA Zhengcai ZHOU Wei & ZOU Liyun .Institute of Immunology PLA Third Military Medical University Chongqing China . College of Bioengineering Chongqing Institute of Technology Chongqing China Correspondence should be addressed to Wu Yuzhang |
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| 作者单位： | Geng Miao,Wu Yuzhang,JIA Zhengcai,ZHOU Wei,ZOU Liyun(Institute of Immunology, PLA Third Military Medical University, Chongqing 400038, China);Lin Zhihua(Institute of Immunology, PLA Third Military Medical University, Chongqing 400038, China;College of Bioengineering, Chongqing Institute of Technology, Chongqing 400050, China) |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金 |
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| 摘 要： | Immunity is one of the functions a body has to iden-tify and eliminate foreign antigens. As one of the surfacemolecules of antigen presenting cells, major histocom-patibility complex (MHC) will exert essential effects dur-ing the antigen presenting procession, and the presenting of MHC-Ⅰ peptide complex is regarded as the prelude to activate the cytotoxic T lymphocytes (CTL) which play an important role in the anti-tumor and anti-virus responses. As we know, CTLs can only identify the co…
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| 收稿时间： | 26 October 2005 |
Screening of HLA-A2 restricted CTL epitopes using molecular simulation |
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| GENG Miao,WU Yuzhang,LIN Zhihua,,JIA Zhengcai,ZHOU Wei & ZOU Liyun .Institute of Immunology,PLA Third Military Medical University,Chongqing ,China, . College of Bioengineering,Chongqing Institute of Technology,Chongqing ,China Correspondence should be addressed to Wu Yuzhang.Screening of HLA-A2 restricted CTL epitopes using molecular simulation[J].Chinese Science Bulletin,2003,48(18):1962-1966. |
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| Authors: | Geng Miao Wu Yuzhang Lin Zhihua Jia Zhengcai Zhou Wei Zou Liyun |
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| Institution: | e-mail: wuyu- zhang@yahoo.com |
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| Abstract: | A new technology has been established to screen CTL epitope by using molecular simulation and molecular dynamics. By using this technology, four peptides of MAGE-2 were screened and further identified by peptide binding assay and cytotoxic assay. Results showed that three candidate peptides had higher affinity to HLA-A2 molecule and were able to pulse CTL activities in vitro in human PBMC. The results indicated that identification of CTL epitopes with computer assistant could overcome the complicated experimental steps, particularly the peptide elution step, and could directly observe peptide-MHC-Ⅰ compound binding pattern and could be used to screen CTL epitopes quickly. |
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| Keywords: | CTL epitope HLA-A2 identification computer assis-tant molecular simulation |
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