| Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon |
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| 作者姓名： | LIU Bin WANG Kemin XIAO Zhiqiang WANG Wei TAN Weihong SUN Yi TANG Hongxing YANG Xiaohai |
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| 作者单位： | [1]State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China; [2]Life Science and Biotechnology Institute, Hunan University, Changsha 410082, China; [3]Chemistry and Chemical Engineer College, Hunan University,Changsha 410082, China [4]Hunan Engineering Research Center for Bio-Nanotechnology, HunanUniversity, Changsha 410082, China [5]Medical Experiment Research Center, Xiangya Hospital, CentralSouth University, Changsha 410008, China [6]Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Changsha 410008, China |
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| 基金项目： | Acknowledgements This work was supported by the National Key Basic Research Program of China （Grant Nos. 2002CB 513110 and 2004CB520804）; the Key Technologies Research and Development Program （Grant No. 2003 BA310A 16）; the International Key Corporation Project of the Chinese Technology Ministry （Grant No. 2003DF000039）; the National Natural Science Foundation of China （Grant No. 20135010）; the Key Project of Natural Science Foundation of Hunan Province （Grant No. 0399Y1006）. |
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| 摘 要： | As an important tumor suppressor, normal expres- sion of ING1 is associated with the maintenance of cell normal physiological functions. Its low expression of- ten causes aberrant events of cell survive, growth and differentiation1] which results in loss…
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| 关 键 词： | ING1 RT-PCR 定量分析 分子信号 mRNA |
| 收稿时间： | 2006-01-17 |
| 修稿时间： | 2006-01-172006-04-18 |
Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon |
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| LIU Bin WANG Kemin XIAO Zhiqiang WANG Wei TAN Weihong SUN Yi TANG Hongxing YANG Xiaohai.Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon[J].Chinese Science Bulletin,2006,51(17):2059-2064. |
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| Authors: | Bin Liu Kemin Wang Zhiqiang Xiao Wei Wang Weihong Tan Yi Sun Hongxing Tang Xiaohai Yang |
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| Institution: | (1) State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, China;(2) Life Science and Biotechnology Institute, Hunan University, Changsha, 410082, China;(3) Chemistry and Chemical Engineer College, Hunan University, Changsha, 410082, China;(4) Hunan Engineering Research Center for Bio-Nanotechnology, Hunan University, Changsha, 410082, China;(5) Medical Experiment Research Center, Xiangya Hospital, Central South University, Changsha, 410008, China;(6) Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Changsha, 410008, China |
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| Abstract: | The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were
quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology.
Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell
line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of
ING1 varied from 7.7 × 10−16 to 53.4 × 10−16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but
also were applied to investigating the interaction of multigenes on signal transduction pathway. |
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| Keywords: | molecular beacon ING1 RT-PCR quantitative detec- tion |
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