| Protein tyrosine phosphatases involved in signaling of the ABA-induced H2O2 generation in guard cells of Vicia faba L. |
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| 作者姓名： | SHIWuliang JIAWensuo LIUXin ZHANGShuqiu |
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| 作者单位： | [1]StateKeyLaboratoryofPlantPhysiologyandBiochemistry,CollegeofBiologicalSciences,ChinaAgriculturalUniversity,Beijing100094,China [2]CollegeofAgronomyandBiotechnology,ChinaAgriculturalUniversity,Beijing100094,China [3]CollegeofLife,LaiyangAgriculturalCollege,Laiyang265200,China |
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| 摘 要： | Although protein tyrosine phosphatases (PTPases) play an important role in signal transduction in animal cells, little is known about the function of PTPases in higher plants. Hydrogen peroxide (H2O2) and mitogen-activated protein kinases (MAPKs) are the critical components of ABA signaling pathway in guard cells. PTPase is an important regulator of MAPK, which is believed to mediate ABA-induced H2O2 generation in guard cells of Vicia faba L. Here, we investigate the possible role of PTPases in stomatal movement process. Phenylarsine oxide (PAO), a specific inhibitor of PTPases, could prevent ABA or H2O2-induced stomatal closure of Vicia faba L; furthermore, it could promote opening of the stomata closed by ABA or H2O2. The activity of PTPases can be effectively inhibited by PAO and H2O2. DTT had no effect on the PAO-induced inhibition of PTPases activity, but it could relieve the inhibition of H2O2 on PTPases activity. PAO could also inhibit the ABA-induced H2O2 generation in guard cells of V‘wia faba L. These results suggested that PTPases is a critical signaling component in ABA-induced stomatal closure, and serve as targets for H2O2 lying on the signaling pathways downstream of ABA induced H2O2 generation.
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| 关 键 词： | 蚕豆属 蛋白质酪氨酸磷酸酶 ABA H2O2 脱落酸 PTP酶 信号转换 |
| 收稿时间： | 2004-05-09 |
| 修稿时间： | 2004-07-29 |
Protein tyrosine phosphatases involved in signaling of the ABA-induced H2O2 generation in guard cells ofVicia faba L. |
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| SHIWuliang JIAWensuo LIUXin ZHANGShuqiu.Protein tyrosine phosphatases involved in signaling of the ABA-induced H2O2 generation in guard cells of Vicia faba L.[J].Chinese Science Bulletin,2004,49(17):1841-1846. |
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| Authors: | Shi Wuliang Jia Wensuo Liu Xin Zhang Shuqiu |
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| Institution: | SHI Wuliang1,JIA Wensuo2,LIU Xin3 & ZHANG Shuqiu1 1.State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100094,China;2.College of Agronomy and Biotechnology,China Agricultural Univer- sity,Beijing 100094,China;3.College of Life,Laiyang Agricultural College,Laiyang 265200,China |
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| Abstract: | Although protein tyrosine phosphatases (PTPases) play an important role in signal transduction in animal cells, little is known about the function of PTPases in higher plants. Hydrogen peroxide (H2O2) and mito- gen-activated protein kinases (MAPKs) are the critical com- ponents of ABA signaling pathway in guard cells. PTPase is an important regulator of MAPK, which is believed to medi- ate ABA-induced H2O2 generation in guard cells of Vicia faba L. Here, we investigate the possible role of PTPases in stomatal movement process. Phenylarsine oxide (PAO), a specific inhibitor of PTPases, could prevent ABA or H2O2-induced stomatal closure of Vicia faba L; furthermore, it could promote opening of the stomata closed by ABA or H2O2. The activity of PTPases can be effectively inhibited by PAO and H2O2. DTT had no effect on the PAO-induced inhi- bition of PTPases activity, but it could relieve the inhibition of H2O2 on PTPases activity. PAO could also inhibit the ABA-induced H2O2 generation in guard cells of Vicia faba L. These results suggested that PTPases is a critical signaling component in ABA-induced stomatal closure, and serve as targets for H2O2 lying on the signaling pathways downstream of ABA induced H2O2 generation. |
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| Keywords: | protein tyrosine phosphatases H2O2 Abscisic acid sig- nal transduction Vicia faba L |
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