| Interaction of APT with BSA or HSA |
| |
| 作者姓名： | CUI Fengling CUI Yanrui LUO Hongxia YAO Xiaojun FAN Jing LU Yan |
| |
| 作者单位： | [1]Henan Key Laboratory for Environmental Pollution Control, School of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China [2]Department of Chemistry, Renmin University of China, Beijing 100872, China [3]Department of Chemistry, Lanzhou University, Lanzhou 730000, China |
| |
| 基金项目： | Acknowledgements This work was supported by the Young Backbone Teacher Support Plan of Henan Colleges and Universities （Grant No. 200470）, the Department of Education of Henan Province （Grant No. 2006150012） and the National Natural Science Foundation of China （Grant Nos. 20443002 and 20575077）. |
| |
| 摘 要： | 1 Introduction In the past few years, a series of saturated, unsatu- rated fatty hydrocarbon and aryl substituted thiourea compounds have been synthesized and successfully used in analytical chemistry1―4]. N-n-amyl-N'-(sodium p-aminobenzenesulfonate) thiourea (APT) is one of these newly synthesized reagents. As a kind of drug, thiourea compounds exhibit various biological activi- ties such as antiviral and antifungal5]. They can be used as insecticides, herbicides and plant-growth regula-…
|
| 关 键 词： | APT BSA 脂肪族烃 牛血清蛋白 HSA 人血清白蛋白 荧光光谱 分子模型 |
| 收稿时间： | 2006-02-09 |
| 修稿时间： | 2006-02-092006-04-17 |
Interaction of APT with BSA or HSA |
| |
| CUI Fengling CUI Yanrui LUO Hongxia YAO Xiaojun FAN Jing LU Yan.Interaction of APT with BSA or HSA[J].Chinese Science Bulletin,2006,51(18):2201-2207. |
| |
| Authors: | Fengling Cui Yanrui Cui Hongxia Luo Xiaojun Yao Jing Fan Yan Lu |
| |
| Institution: | (1) Henan Key Laboratory for Environmental Pollution Control, School of Chemistry and Environmental Science, Henan Normal University, Xinxiang, 453007, China;(2) Department of Chemistry, Renmin University of China, Beijing, 100872, China;(3) Department of Chemistry, Lanzhou University, Lanzhou, 730000, China |
| |
| Abstract: | In this work, N-n-amyl-N'-(sodium p- aminobenzenesulfonate) thiourea (APT) containing saturated fatty hydrocarbon group was synthesized. Fluorescence quenching methods in combination with UV absorption spectra and molecule modeling method were used to study the interaction between APT and bovine serum albumin (BSA) or human se-rum albumin (HSA). The binding constants of APT with BSA or HSA were determined at different tem-peratures under the optimum conditions based on the fluorescence quenching results. The binding characteristics of APT and BSA or HSA were reported and the binding sites were obtained. The binding mode was suggested to be mainly hydrophobic interaction, which was consistent with molecular modeling study. |
| |
| Keywords: | N-n-amyl-N-(sodium p-aminobenzenesulfonate) thiourea(APT) bovine serum albumin(BSA) human serum albumin (HSA) fluorescence spectroscopy molecular model |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
