| Probing molecular interaction between transferrin and anti-transferrin by atomic force microscope |
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| 作者姓名： | ZHENG Zhiwen YANG Peihui ZENG Gucheng CAI Jiye |
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| 作者单位： | [1]Key Laboratory of Specially Functional Materials and AdvancedManufacturing Technology, Ministry of Education, South China University of Technology, Guangzhou 510640, China [2]Department of Chemistry, Jinan University, Guangzhou 510632, China |
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| 摘 要： | ATOMIC FORCE MICROSCOPY(AFM)IS A POWERFUL TOOL FOR STUDYING BIOMACROMOLECULAR STRUCTURE AND THEIR IN-TERACTION WITH A RESOLUTION AT THE NANOMETER LEVEL IN PHYSIOLOGY-LIKE SITUATION.THE INTERACTIONS BETWEEN LIGAND AND RECEPTOR,ANTIGEN AND ANTIBODY,AND INTE…
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| 关 键 词： | 转铁球蛋白 抗转铁球蛋白 分子间相互作用 原子力显微镜 生物大分子 |
| 收稿时间： | 2005-04-19 |
| 修稿时间： | 2005-04-192005-06-20 |
Probing molecular interaction between transferrin and anti-transferrin by atomic force microscope |
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| ZHENG Zhiwen YANG Peihui ZENG Gucheng CAI Jiye.Probing molecular interaction between transferrin and anti-transferrin by atomic force microscope[J].Chinese Science Bulletin,2006,51(4):405-408. |
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| Authors: | Zhiwen Zheng Peihui Yang Gucheng Zeng Jiye Cai |
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| Institution: | (1) Key Laboratory of Specially Functional Materials and Advanced Manufacturing Technology, Ministry of Education, South China University of Technology, Guangzhou, 510640, China;(2) Department of Chemistry, Jinan University, Guangzhou, 510632, China |
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| Abstract: | The interaction between transferrin (Tf) and its antibody was investigated by atomic force microscope. Tf-antibody was immobilized on the Au-coated glass slide, and the specific combination between antibody and antigen was also character-ized by AFM. The results showed that holo-transferrin was jogged with anti-transferrin, and binded anti-tran- sferrin more tightly than apo-transferrin. The force- distance curves revealed that the affinity of anti-trans- ferrin and holo-transferrin was much stronger than that of apo-transferrin. |
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| Keywords: | holo-transferrin apo-transferrin anti-transferrin AFM |
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