| 苦杏仁苷与人血清白蛋白相互作用的荧光光谱研究 |
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| 引用本文： | 何丽君;霍彩霞;徐飞;李生英;魏云霞;程小丽.苦杏仁苷与人血清白蛋白相互作用的荧光光谱研究[J].甘肃教育学院学报(自然科学版),2013(5):55-57. |
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| 作者姓名： | 何丽君;霍彩霞;徐飞;李生英;魏云霞;程小丽 |
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| 作者单位： | 兰州城市学院化学与环境科学学院 |
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| 基金项目： | 甘肃省城市发展研究院科研项目(2012-GSCFY-KJ04) |
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| 摘 要： | 应用荧光光谱法,研究了苦杏仁苷与人血清白蛋白(HSA)在pH=7.40的Tris-HCl缓冲溶液中相互作用的情况.研究表明,苦杏仁苷通过静态机理猝灭了HSA的内源荧光.并计算得到苦杏仁苷与HSA的结合常数和结合位点数.通过热力学数据,推断出苦杏仁苷与人血清白蛋白之间主要靠氢键和范德华力结合.同时采用同步荧光技术考察了苦杏仁苷对HSA构象的影响.
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| 关 键 词： | 苦杏仁苷 人血清白蛋白 相互作用 荧光光谱 |
Study on the Interaction between Amygdalin and Human Serum Albumin by Fluorescence Spectroscopy |
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| Institution: | HE Li-jun;HUO Cai-xia;XU Fei;LI Sheng-ying;WEI Yun-xia;CHENG Xiao-li;School of Chemistry and Environmental Science,Lanzhou City University; |
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| Abstract: | The interaction between amygdalin and human serum albumin(HSA)in buffer solution(pH =7.40)were studied by fluorescence spectroscopy.It was found that amygdalin quenched the fluorescence of HSA via a static quenching process.The binding constant and number of binding sites were found.Values of thermodynamic parameters were calculated.These dates indicated that hydrogen bonds and van der Waals force played a major role in the binding of amygdalin and HSA.Using synchronous fluorescence spectroscopy,the effect of amygdalin on the configuration of HSA was studied. |
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| Keywords: | amygdalin human serum albumin interaction fluorescence spectroscopy |
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