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原生质体诱变选育分解X光胶片的菌株
引用本文:陈碧娥,王丽娜.原生质体诱变选育分解X光胶片的菌株[J].华侨大学学报(自然科学版),1992,13(1):125-128.
作者姓名:陈碧娥  王丽娜
作者单位:华侨大学化工与生化工程系,华侨大学化工与生化工程系
摘    要:从厦门福达感光胶片厂的污水中分离到207株芽孢杆菌,其中一株显示了较强的分解明胶的能力.该菌经鉴定为巨大芽孢杆菌(Bucillus megaterium 207).用溶菌酶制备207菌的原生质体,经紫外线诱变获得一变异株(207—A5),产蛋白酶活力由830u/ml 提高到4731u/ml.

关 键 词:原生质体  蛋白酶  感光乳剂    回收

Selective Breeding of a Bacterial Strain Capable of Decomposing X-Ray Film by Mutagenizing the Protoplast
Chen Bie Wang Lina.Selective Breeding of a Bacterial Strain Capable of Decomposing X-Ray Film by Mutagenizing the Protoplast[J].Journal of Huaqiao University(Natural Science),1992,13(1):125-128.
Authors:Chen Bie Wang Lina
Institution:Department of Chemical and Biochemical Enginnering
Abstract:207 strains of bacilli were isolated from the sewage samples of Fuda Film Factory in Xia- men.One of these strains showed a higher capablity of decomposing gelatin.The strain was identified as Bacillus Megaterium 207.Amutant,namely 207-A5 ,can be obtained by preparing the protoplast of B. Megaterium 207 with lysozyme and mutagemizing it with UV light.207-A5 is capable of rising the aci- tivity of producing protease from 830 u/ml to 4,731 u/ml.
Keywords:Protoplast  mutagenize  Protease
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