| 荧光探针自组装膜对久效磷农药的检测 |
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| 引用本文： | 杨光昕,孙向英,刘斌.荧光探针自组装膜对久效磷农药的检测[J].华侨大学学报(自然科学版),2010,31(1). |
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| 作者姓名： | 杨光昕 孙向英 刘斌 |
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| 作者单位： | 华侨大学,材料科学与工程学院,福建,泉州,362021 |
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| 基金项目： | 国家自然科学基金资助项目(20575023);;福建省国际合作重点项目(2006I0021);;福建省自然科学基金资助项目(D0710017,D0810016,U0850008);;教育部留学回国人员科研启动项目(2008年度) |
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| 摘 要： | 采用CdSe量子点作为荧光探针,将巯基丙酸稳定的CdSe、聚二烯丙基二甲基氯化铵(PDDA)和乙酰胆碱酯酶(ACHE)通过静电吸引法,层层组装到石英表面,构建一种荧光性自组装多层膜Quartz/PDDA/CdSe/PDDA/ACHE/PDDA.利用有机磷农药对乙酰胆碱酯酶的抑制机理,成功地将自组装膜用于痕量久效磷农药的检测.实验表明,在乙酰胆碱酯酶被抑制16 min,底物乙酰胆碱浓度为8.8 mmol.L-1的条件下,该膜对久效磷的检测限低至48.64 nmol.L-1.所构建的自组装膜稳定性较好、灵敏度较高,可以再生使用.
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| 关 键 词： | 量子点 荧光探针 乙酰胆碱酯酶 自组装膜 有机磷农药 |
Determination of Monocrotophos Based on Self-Assembled Multilayer Films with Fluorescent Probes |
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| YANG Guang-xin,SUN Xiang-ying,LIU Bin.Determination of Monocrotophos Based on Self-Assembled Multilayer Films with Fluorescent Probes[J].Journal of Huaqiao University(Natural Science),2010,31(1). |
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| Authors: | YANG Guang-xin SUN Xiang-ying LIU Bin |
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| Institution: | College of Materials Science and Eenginnering;Huaqiao University;Quanzhou 362021;China |
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| Abstract: | A fluorescent self-assembled multilayer films(Quartz/PDDA/CdSe/PDDA/ACHE/PDDA) was constructed by putting3-mercaptopropionic acid modified CdSe quantum dots(QDs),PDDA and acetylcholinesterase(ACHE) onto the quartz substrate alternately with the layer by layer assembly technique.And CdSe QDs on the films was used as fluorescent probes.By the enzymatic inhibition,the self-assembled multilayer films could be used to detect trace organophosphorus pesticides successfully.This method was used to detect monocrotop... |
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| Keywords: | quantum dots fluorescent probe acetylcholinesterase self-assembled multilayers films organophosphorus pesticide |
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