| 产几丁质酶菌株的分离与鉴定及产酶条件优化 |
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| 引用本文： | 李红然,贺淹才,刘治江,刘杨斐.产几丁质酶菌株的分离与鉴定及产酶条件优化[J].华侨大学学报(自然科学版),2009,30(5). |
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| 作者姓名： | 李红然 贺淹才 刘治江 刘杨斐 |
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| 作者单位： | 华侨大学,工业生物技术福建省高等学校重点实验室,福建,泉州,362021 |
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| 基金项目： | 福建省自然科学基金资助项目 |
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| 摘 要： | 从土壤中分离到一株产几丁质酶的细菌LL-C.该菌经16S rDNA序列同源性分析及形态培养特征、生理生化特征分析,鉴定为Luteibacter rhizovicinus,属于黄单胞菌科(Xanthomonadaceae).以不同发酵时间、碳源、氮源、起始pH值、培养基装液量和培养温度为因素,考察LL-C菌株产几丁质酶的优化条件.结果表明,最有利于该菌产几丁质酶的条件:碳源为胶体几丁质、氮源为(NH4)2SO4,培养基起始pH值为4.5,培养基装液量为30 mL,培养温度为32 ℃,振荡培养时间为7 d,此优化条件下,摇瓶产酶活力为115.02 μkat·L-1.
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| 关 键 词： | 几丁质酶 优化 酶活力 |
Isolation,Identification of Chitinase Producing Microorganism and Optimization of Its Fermentation Conditions |
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| LI Hong-ran,HE Yan-cai,LIU Zhi-jiang,LIU Yang-fei.Isolation,Identification of Chitinase Producing Microorganism and Optimization of Its Fermentation Conditions[J].Journal of Huaqiao University(Natural Science),2009,30(5). |
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| Authors: | LI Hong-ran HE Yan-cai LIU Zhi-jiang LIU Yang-fei |
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| Institution: | Key Laboratory of Industrial Biotechnology of Fujian Drovince University;Huaqiao University;Quanzhou 362021;China |
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| Abstract: | A chitinase producing strain LL-C was isolated from soil.According to the analysis of the 16S rDNA sequence,the cultivation condition,its morphology and physiological characteristics of the LL-C,it was identified as Luteibacter rhizovicinus belong to Xanthomonadaceae.The effects of a range of factors on the production of chitinase of the LL-C were studied,including time,carbon source,nitrogen source,pH,volume of medium and cultural temperature.Experimental results showed that the optimal carbon and nitrogen... |
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| Keywords: | Luteibacter rhizovicinus |
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