促进种子萌发的重楼内生菌的筛选 |
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引用本文: | 宋发军,于鹏飞,刘 佳,席晓圆,赵盛英,张 鹏.促进种子萌发的重楼内生菌的筛选[J].中南民族大学学报(自然科学版),2015(3):29-32. |
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作者姓名: | 宋发军 于鹏飞 刘 佳 席晓圆 赵盛英 张 鹏 |
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作者单位: | 中南民族大学 生命科学学院, 生物技术国家民委重点实验室/武陵山区特色资源植物种质保护与利用湖北省重点实验室,武汉 430074 |
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基金项目: | 国家自然科学基金资助项目(31370118);中南民族大学2014年硕士研究生学术创新基金(2014sycxjj112) |
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摘 要: | 通过比较60株重楼内生细菌发酵液的水相提取液和有机相提取液对冬小麦种子萌发的影响,发现8株细菌的有机相提取物具有促萌发作用.将其中4株细菌的发酵液依次用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取,蒸干物用甲醇溶解并稀释成不同浓度处理冬小麦种子.结果表明:肠杆菌属74#菌的石油醚相提取物、不动杆菌属108#菌的氯仿相提取物、芽孢杆菌属138#菌的氯仿相提取物和克雷白氏杆菌属149#菌的乙酸乙酯相提取物的促萌作用较强(p0.05);其中芽孢杆菌属138#菌的氯仿相提取物的1/2稀释液的促萌作用最强(p0.01).
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关 键 词: | 重楼内生菌 冬小麦种子 萌发 提取物 |
Screening of Polyphylla Endophytes with Germination Effect |
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Abstract: | The effect of the aqueous and organic extracts of the fermentation broths of 60 Polyphylla endophytic bacteria on the germination of winter wheat were compared. The organic extracts of 8 endophytes had germination effect and four fermentation broths from them were then extracted by petroleum ether, chloroform, ethyl acetate and n-butanol, respectively. The dried product was dissolved with methanol and diluted into different concentrations to treat the winter wheat seeds. The results showed that the petroleum-ether extract from Enterobacter sp. 74#, the chloroform extract from Acinetobacter sp. 108# , the chloroform extract from Bacillus sp. 138# and the ethyl acetate extract from Klebsiella sp. 149# had significant germination effect (p <0.05). The 1/2 dilution of the chloroform extract from Bacillus sp. 138# had the best germination effect (p <0.01). |
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Keywords: | Polyphylla endophyte winter wheat seeds germination extract |
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