| 气-质联用法研究水溶性茯苓多糖提取方法 |
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| 引用本文： | 王献,赵文杰,苏波.气-质联用法研究水溶性茯苓多糖提取方法[J].中南民族大学学报(自然科学版),2010,29(1):28-31. |
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| 作者姓名： | 王献 赵文杰 苏波 |
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| 作者单位： | 中南民族大学,化学与材料科学学院,武汉,430074 |
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| 基金项目： | 国家自然科学基金资助项目，教育部留学回国人员科研启动基金资助项目，武汉市晨光计划资助项目，中南民族大学自然科学基金资助项目 |
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| 摘 要： | 为研究水溶性茯苓多糖提取方法,分别采用热水提取法及超声提取法提取水溶性茯苓多糖,并以杂多糖的得率为指标设计正交试验,采用BSTFA(双(三甲基硅烷基)三氟乙酰胺)和DM F(N,N-二甲基甲酰胺)对杂多糖进行衍生化,采用D-果糖作内标进行气相色谱-质谱联用(GC-M S)定量分析.实验表明:热水浸提的最佳条件为固液比1∶40,提取时间3 h,提取温度90℃;超声波法最佳条件为固液比1∶40,提取时间60 m in,超声功率为100 W.热水提取和超声提取水溶性茯苓多糖的平均提取率分别为13.0%,8.6%,热水浸提法的多糖得率较高.均高于现有文献值报道.
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| 关 键 词： | 气-质联用 热水浸提法 超声波提取法 水溶性茯苓多糖 |
Study on Extraction Methods of Water-Soluble Pachymaran by GC-MS |
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| Wang Xian,Zhao Wenjie,Su Bo.Study on Extraction Methods of Water-Soluble Pachymaran by GC-MS[J].Journal of South-Central Univ for,2010,29(1):28-31. |
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| Authors: | Wang Xian Zhao Wenjie Su Bo |
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| Institution: | Wang Xian,Zhao Wenjie,Su Bo(College of Chemistry , Materials Science,South-Central University for Nationalities,Wuhan 430074,China) |
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| Abstract: | This work has inverstigated the extraction methods of water soulble pachymaran and qualitatively and quantitatively determined poly sacchairide components of Poria cocos F.A.Wolf by gas chromatography-mass spectrometry(GC-MS).Water-soluble pachymaran was extracted by hot water and ultrasound,respectively.The optimum extraction conditions were obtained by a series of orthogonal tests in which heteropolysaccharide yield was used as an indicator.STFA(bis-(trimethylsilyl)trifluoroacetamide) was used as the deri... |
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| Keywords: | gas chromatography-mass spectrometry(GC-MS) hot water extraction ultrasonic extraction water-soluble pachymaran1 |
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