| 人面果酮抗糖尿病活性的筛选 |
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| 引用本文： | 王朝元,田世婷,陈 玉.人面果酮抗糖尿病活性的筛选[J].中南民族大学学报(自然科学版),2015(2):23-27. |
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| 作者姓名： | 王朝元 田世婷 陈 玉 |
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| 作者单位： | 1 中南民族大学 生命科学学院，武汉430074；2 中南民族大学 化学与材料科学学院，武汉 430074 |
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| 基金项目： | 国家自然科学基金资助项目(31370379) |
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| 摘 要： | 为研究人面果中酮的抗糖尿病活性,筛选具有较强活性的化合物,采用MTT法检测了化合物对HepG2细胞增殖率的影响.用高浓度胰岛素诱导HepG2细胞,建立了胰岛素抵抗模型(HepG2/IR),并采用葡萄糖氧化酶法,检测了不同浓度化合物对HepG2细胞胰岛素抵抗模型的葡萄糖消耗的影响.结果表明:人面果中酮中化合物1、3、4(除化合物2、5)能够明显促进HepG2/IR细胞的葡萄糖消耗,具有潜在的抗糖尿病活性.
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| 关 键 词： | 人面果 酮 胰岛素抵抗 葡萄糖消耗 |
Screening of the Anti-diabetic Activities of Xanthones from Garcinia xanthochymus |
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| Wang Chaoyuan;Tian Shiting;Chen Yu.Screening of the Anti-diabetic Activities of Xanthones from Garcinia xanthochymus [J].Journal of South-Central Univ for,2015(2):23-27. |
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| Authors: | Wang Chaoyuan;Tian Shiting;Chen Yu |
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| Institution: | Wang Chaoyuan;Tian Shiting;Chen Yu;College of Life Sciences,South-Central University for Nationalities;College of Chemistry and Materials Sciences,South-Central University for Nationalities; |
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| Abstract: | To study the anti-diabetic activities of xanthones from Garcinia xanthochymus and to screen the active compounds, MTT assay was used to determine its effect on the survival rate of HepG2 cells. The HepG2 cells were induced by high concentration of insulin to establish the insulin-resistance cell model (HepG2/IR). Then the glucose consumption of cells treated with different concentration of compounds was measured by glucose oxidase and peroxidase (GOD-POD) assay. The results showed that compounds 1, 3, and 4 (except compounds 2 and 5) of the xanthones from Garcinia xanthochymus could significantly enhance the glucose consumption of HepG2/IR cell, which suggested that they had the potential anti-diabetic activities. |
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| Keywords: | Garcinia xanthochymus xanthone insulin-resistant glucose consumption |
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