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| 中药"龙泉复方"的不同配伍对肿瘤细胞系HepG-2和EC-1增殖的影响 | |
| 引用本文： | 李劲,邓志芳,高雯琪,胡文娟,刘莹,姚品芳,李广灿,陈焕朝,梅之南.中药"龙泉复方"的不同配伍对肿瘤细胞系HepG-2和EC-1增殖的影响[J].中南民族大学学报(自然科学版),2011,30(1):46-49. |
| 作者姓名： | 李劲 邓志芳 高雯琪 胡文娟 刘莹 姚品芳 李广灿 陈焕朝 梅之南 |
| 作者单位： | 1. 中南民族大学,生命科学学院,武汉,430074 2. 湖北省肿瘤医院,武汉,430079 3. 中南民族大学,药学院,武汉,430074 |
| 基金项目： | 国家民委自然科学基金资助项目，湖北省卫生厅医学科研基金资助项目 |
| 摘 要： | 通过MTT(四甲基偶氮唑盐)法绘制细胞增殖曲线观察药物对肝癌细胞系HepG-2和食管鳞癌细胞系EC-1细胞增殖的影响,用荧光染色法进一步确定"龙泉复方"有效成分诱导细胞凋亡的较佳配伍及其有效作用浓度范围.结果表明:对抑制HepG-2细胞增殖有效作用的浓度:七味单方(马钱子、山慈菇、喜树果、龙葵、石上柏、青黛、紫菀)浸膏... |
| 关 键 词： | 龙泉复方 配伍 药物浓度 肿瘤细胞 MTT(四甲基偶氮唑盐)法 AO/EB染色法 |
Different Compatibility of Chinese Medicine "Longquan Compound" Effect on HepG-2 and EC-1 Cell Line Proliferation |
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| Li Jin,Deng Zhifang,Gao Wenqi,Hu Wenjuan,Liu Ying,Yao Pinfang,Li Guangcan,Chen Huanchao,Mei Zhinan.Different Compatibility of Chinese Medicine "Longquan Compound" Effect on HepG-2 and EC-1 Cell Line Proliferation[J].Journal of South-Central Univ for,2011,30(1):46-49. | |
| Authors: | Li Jin Deng Zhifang Gao Wenqi Hu Wenjuan Liu Ying Yao Pinfang Li Guangcan Chen Huanchao Mei Zhinan |
| Institution: | Li Jin1,Deng Zhifang1,Gao Wenqi1,Hu Wenjuan1,Liu Ying1,Yao Pinfang3,Li Guangcan3,Chen Huanchao3,Mei Zhinan2(1 College of Life Science,South-Central University for Nationalities,Wuhan 430074,China,2 College of Pharmacy,3 Hubei Cancer Hospital,Wuhan 4300749,China) |
| Abstract: | The cell proliferation curve and observation on the effect of the cells of HepG-2 and EC-1 caused by the drugs were obtained through the method of MTT(methyl thiazolyl tetrazolium),and the optimal compatibility and concentration of the drugs were determined by fluorescence staining.It is shown that the seven-mixed drug(the major effective components extracted from strychnine,mountain keats mushroom,common camptotheca fruit,solanum nigrum,stone cypress,natural indigo and tatarian aster root) and solanum nigr... |
| Keywords: | Longquan compound compatibilities concentration MTT HepG-2 and EC-1 cell AO/EB staining |
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