| 链霉菌H197 mtg基因毕赤酵母表达载体的构建与鉴定 |
| |
| 引用本文： | 何冬兰,王亚南,邵坤彦,叶程.链霉菌H197 mtg基因毕赤酵母表达载体的构建与鉴定[J].中南民族大学学报(自然科学版),2012,31(1):30-33. |
| |
| 作者姓名： | 何冬兰 王亚南 邵坤彦 叶程 |
| |
| 作者单位： | 中南民族大学生命科学学院,武汉,430074 |
| |
| 基金项目： | 湖北省自然科学基金资助项目(2010CDZ046) |
| |
| 摘 要： | 将酵母胞内表达质粒pPIC3.5k重组成带有谷氨酰胺转胺酶的新型表达载体pPIC3.5k/MTG,为谷氨酰胺转胺酶（MTG）在毕赤酵母中的表达研究提供了实验基础.利用PCR的方法扩增出MTG基因片段,将目的片段和质粒pPIC3.5k进行双酶切后进行连接,构成重组质粒,然后转化至E.coli DH5α感受态细胞中,筛选阳性克隆,经测序证明为目的基因片段.结果表明：经过PCR和酶切均证明已经将目的片段转到酵母表达载体pPIC3.5k内.
|
| 关 键 词： | 谷氨酰胺转胺酶 表达 载体 构建 |
Construction and Identification of the Pichia pastoris Expression Vector for mtg Gene in Streptomyces sp. H197 |
| |
| He Donglan,Wang Ya′nan,Shao Kunyan,Ye Cheng.Construction and Identification of the Pichia pastoris Expression Vector for mtg Gene in Streptomyces sp. H197[J].Journal of South-Central Univ for,2012,31(1):30-33. |
| |
| Authors: | He Donglan Wang Ya′nan Shao Kunyan Ye Cheng |
| |
| Institution: | (College of Life Science,South-Central University for Nationalities,Wuhan 430074,China) |
| |
| Abstract: | To reconstruct the Pichia pastoris expression vector pPIC3.5k/MTG with pPIC3.5k,providing the basis of MTG expressing in Pichia pastoris,the target fraction was amplified by PCR and connected to pPIC3.5k.The recombinant plasmid pPIC3.5k/MTG was transformed into E.coli DH5α.The recombinant E.coli DH5α/pPIC3.5k-MTG was screened in LB/Amp plates,and identified by sequencing,PCR and restriction enzyme digestion.The result showed that the Pichia pastoris expression vector contained mtg gene. |
| |
| Keywords: | transglutaminase(MTG) expression vector construction |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中南民族大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《中南民族大学学报(自然科学版)》下载免费的PDF全文 |
|
