苦物质引起去甲肾上腺素预收缩的大鼠主动脉平滑肌舒张的作用机理研究

为研究苦物质舒张预收缩的大鼠胸主动脉平滑肌的作用机制,以测定肌张力为主要手段,结合多种细胞信号通路阻断剂研究了苦物质的作用通路.结果表明:苦物质对于去甲肾上腺素介导的大鼠胸主动脉的收缩具有高效、快速的舒张作用,此舒张作用与IP3受体和L型钙通道的阻断密切相关.故以氯喹和苦精为代表的苦物质因对血管平滑肌具有良好的舒张作用,有望成为针对高血压等血管相关疾病的新的治疗药物.

Relaxation Mechanism of Bitter Tastants on Rat Thoracic Aorta Precontracted by Norepinephrine

To elucidate the relaxation mechanism of bitter tastant on precontracted rat thoracic aorta, the effect of bitter tastants on muscle tension in rat thoracic aorta was investigated, combined with the application of a variety of cell signaling pathway blockers. The results indicated that bitter tastants induced an effective and fast relaxation of rat thoracic aorta precontracted by norepinephrine, which was closely related to the blocking of IP3 receptor and L-type calcium channels. Thus bitter tastants represented by chloroquine and denatonium had good diastolic effect on vascular smooth muscle and had the potential to be used for blood vessel related diseases such as hypertension.