中药大黄抗柯萨奇病毒作用的实验研究

采用大黄1～4#有效提取部位进行了体外抗柯萨奇病毒B3(CVB3)的研究.通过观察病毒引起的细胞病变效应(CPE)、MTT法检测细胞活性,作为考核药物抗病毒作用.结果表明:大黄1～4#有效提取部位对CVB3无直接灭活作用,也不能阻止CVB3的吸附,而能抑制CVB3在Hep-2细胞内的的生物合成,其半数抑制浓度(IC50)分别为34.7、33.0、33.9、34.3 mg/L,治疗指数(TI)分别为7.2、7.6、7.6、7.2.与病毒唑(IC50=30.6 mg/L,TI=6.6,P＞0.01)相当,优于大黄提取液(IC50=48.5 mg/L,TI=4.7,P＜0.01).在2.5～120 mg/L范围内1～4#部位与CVB3抑制率呈明显的量效关系(P＜0.01),120 mg/L时能完全抑制CVB3在Hep-2细胞内的增殖.所以大黄1～4#有效提取部位安全高效地抑制CVB3在Hep-2细胞中的增殖.

Antiviral Activity of the Extracts from Rheum Officinale against Coxackievirus B3 Virus in Vitro

To observe the antiviral activity of the effective extracts No.1,2,3,4 from Rheum officinale against coxackievirus B_3 virus,cell culture technigue was used to observe cytopathic effect(CPE) and MTT colorimetric assay were used to get virus inhibitory rate.The results showed that the four extracts may inhibit CVB_3 biological synthesis other than directly inactivate this virus or block its adsorption to susceptible cells.The inhibitory concentration(IC_(50)) of the four extracts were 34.7,33.0,33.9 and 34.3 mg/L respectively.And the treatment index(TI) were relatively figured out as 7.2,7.6,7.6 and 7.2.This effect is similar to that of virazole at the same dosage level(IC_(50)=30.6 mg/L,TI=6.6,P>0.01).And more effective than the compounded liquid from Rheum officinale(IC_(50)=48.5 mg/L,TI=4.7,P<0.01).And there are obvious dosage-effect relationship between the 2.5~120 mg/L dose of No.1,2,3,4 and the anti-CVB_3 inhibitory rate in vitro(P<0.01).120 mg/L of the extracts No.1~4 can completely inhibit the proliferation of CVB_3 in Hep-2 cells.So we conclude that the extracts No.1,2,3,4 from Rheum officinale can inhibit the profication of the CVB_3 in Hep-2 cell effectively and safely.