| 水稻碱性几丁质酶基因表达载体pCAMBIA1301-RC24的构建 |
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| 引用本文： | 高丽美,李永锋,徐子勤.水稻碱性几丁质酶基因表达载体pCAMBIA1301-RC24的构建[J].山西师范大学学报,2009,23(3):82-86. |
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| 作者姓名： | 高丽美 李永锋 徐子勤 |
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| 作者单位： | 高丽美,李永锋(山西师范大学生命科学学院,山西,临汾,041004);徐子勤(西北大学生命科学学院生物技术省级重点实验室,陕西,西安,710069) |
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| 摘 要： | 用限制性内切酶Kpn1从质粒pYA024中切出大小约2．75kb的片段，此片段包含Actin1启动子、NOS终止子、水稻几丁质酶基因．将其插入到表达载体pCAMBIA1301-imp的多克隆位点内，构建成了水稻碱性几丁质酶基因的表达载体．利用液氮冻融法将构建好的表达载体导入发根农杆菌LBA4404中，以用于生物工程下游技术研究．
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| 关 键 词： | 质粒pYAO24 几丁质酶基因 表达载体pCAMBIA1301-imp 冻融法 发根农杆菌 |
Construction of the Expression Vector pCAMBIA 1301-RC24 Carrying Chitinase Gene |
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| Institution: | GAO Li-mei, LI Yong-feng , XU Zi-qin ( 1. College of Life Science, Shanxi Normal University, Linfen 041004, Shanxi, China ; 2. Provicial Key Bio-technology Laboratory, Northern West University, Xi＇an 710069, Shaanxi, China) |
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| Abstract: | A 2.75 kb fragment carrying the chitinase gene from rice,Actinl promoter and Nos interminator was isolated by Kpnl from plasmid pY024, then inserted into the many cloning sites （MCS） in the expression vector pCAMBIA1301-imp, yield the expression vector of chitinase gene from rice-pCAMBIA1301-RC24. And the expression vector pCAMBIA1301-RC24 was transformed into Agrobacterium rhizogenes by using freeze-thaw method, in order to using in bio-enginering technology. |
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| Keywords: | plasmid pYAO24 chitinase gene expression vector pCAMBIA1301 -imp freeze-thaw agrobacterium rhizogene |
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