| 高磁性壳聚糖微粒的制备与应用 |
| |
| 引用本文： | 安小宁,苏致兴.高磁性壳聚糖微粒的制备与应用[J].兰州大学学报(自然科学版),2001,37(2):100-104. |
| |
| 作者姓名： | 安小宁 苏致兴 |
| |
| 作者单位： | 兰州大学化学化工学院, |
| |
| 基金项目： | 南开大学吸附分离功能高分子材料国家重点实验室资金! (9919)资助项目 |
| |
| 摘 要： | 采用壳聚糖包埋磁粉，经戊二醛修饰、环氧氯丙烷交联制得高磁性壳聚糖微料。此微粒共价结合卵清粘蛋白得到磁性亲和吸附剂，可应用于胰蛋白酶的亲和纯化。
|
| 关 键 词： | 高磁性壳聚糖微粒 亲和吸附剂 胰蛋白酶 亲和纯化 制备 共价偶联 卵清粘蛋白 |
| 文章编号： | 0455-2059(2001)02-0100-05 |
| 修稿时间： | 2000年6月24日 |
Preparation and Application of Chitosan Particles with High Magnetic Properties |
| |
| An Xiaoning,Su Zhixing.Preparation and Application of Chitosan Particles with High Magnetic Properties[J].Journal of Lanzhou University(Natural Science),2001,37(2):100-104. |
| |
| Authors: | An Xiaoning Su Zhixing |
| |
| Abstract: | Chitosan particles with high magnetic properties were prepared from chitosan coated on magnetic powder, glutaraldehyde modified and then epichlorohydrin cross linked. The magnetic properties, the surface and the morphology of the magnetic particles were charac terized. An optimum condition of immobilization of ovomucoid on magnetic particles was obtained, then these particles were used for affinity purification of trypsin, the rate of recovery activity being 41.2%, the purification time being 1.56. The proposed method was applied successfully to the purification of trypsin. |
| |
| Keywords: | chitosan particles with high magnetic properties ovomucoid immobilization affinity purification of trypsin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
