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根癌农杆菌介导的马铃薯遗传转化体系的研究
引用本文:郑秀芳,缪为,李名扬.根癌农杆菌介导的马铃薯遗传转化体系的研究[J].兰州大学学报(自然科学版),2005,41(3):41-44.
作者姓名:郑秀芳  缪为  李名扬
作者单位:1. 河西学院,生物系,甘肃,张掖,734000
2. 重庆市发展与改革委员会,重庆,400000
3. 西南农业大学,园艺系,重庆,北陪,400716
摘    要:以重庆主栽品种的叶片和茎段为外植体,研究了不同转化条件(菌液浓度、感染时间、预培养及共培养时间)对转化效率的影响,以及选择压和培养基对抗性愈伤出芽的影响,初步建立了马铃薯品种"台湾红"和"Spunta"的遗传转化体系并获得了转化植株.

关 键 词:马铃薯  遗传转化  根癌农杆菌
文章编号:0455-2059(2005)03-0041-04

Genetic transformation mediated by Agrobactium tumefaciens in potato (Solanum tuberosum L)
ZHENG Xiu-fang,MIAO Wei,LI Ming-yang.Genetic transformation mediated by Agrobactium tumefaciens in potato (Solanum tuberosum L)[J].Journal of Lanzhou University(Natural Science),2005,41(3):41-44.
Authors:ZHENG Xiu-fang  MIAO Wei  LI Ming-yang
Abstract:Leaf disks and stem segments from potato cultivars of Chongqing were used as receptor materials to study the effects of different cultivars, explants and different transformation conditions (the concentration of A. tumefaciens, infecting period, length of preculture and coculture) on transformation effencies, and the effects of kannamycin concentration and media on resistant callus shooting. Transformation systems for two potato cultivars "Taiwanhong" and "Spunta" were preliminarily set up and the transgenic plants were alse obtained.
Keywords:potato  transformation  Agrobactium tumefaciens
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