|
|||||
|
|
| 缩氨基硫脲类钯抗癌配合物初步筛选与作用机理的荧光法研究 | |
| 引用本文： | 王敏,徐志栋,王流芳,曲东明.缩氨基硫脲类钯抗癌配合物初步筛选与作用机理的荧光法研究[J].兰州大学学报(自然科学版),2001,37(2):82-86. |
| 作者姓名： | 王敏 徐志栋 王流芳 曲东明 |
| 作者单位： | 1. 兰州大学 2. 河北科技大学化学与制药工程学院, 3. 白求恩医学院药学系, |
| 基金项目： | 国家科委生命科学技术发展中心(96-901-05-128)和新药研究国家重点实验室基金资助项目. |
| 摘 要： | 新合成了8-乙酰基-4-甲基-7羟基香豆素缩氨基硫脲、3-乙酰基-7-羟基香豆素缩氨基硫脲及其与钯的配合物，以溴化乙锭为荧光探针，研究了配合物与小牛胸腺DNA的作用机制，初步判定了配合物的抗癌活性，计算了与DNA的结合常数。研究表明荧光筛选法结果与体外筛选的四唑盐染色法结果基本一致。 |
| 关 键 词： | 荧光筛选法 钯 配合物 抗癌活性 作用机制 缩氨基硫脲类 抗癌药物 |
| 文章编号： | 0455-2059(2001)02-0082-05 |
| 修稿时间： | 2000年4月17日 |
Fluorescence Method Used for Preliminary Screening and Study on Mechanism of the Interaction of Thiosemicarbazone and Palladium Complexes as Potential Antitumor Agents |
|
| Wang Min ,Xu Zhidong,Wang Liufang,Qu Dongming.Fluorescence Method Used for Preliminary Screening and Study on Mechanism of the Interaction of Thiosemicarbazone and Palladium Complexes as Potential Antitumor Agents[J].Journal of Lanzhou University(Natural Science),2001,37(2):82-86. | |
| Authors: | Wang Min Xu Zhidong Wang Liufang Qu Dongming |
| Institution: | Wang Min 1,2,Xu Zhidong3,Wang Liufang1,Qu Dongming4 |
| Abstract: | Two new palladium complexes of 8 acetyl 4 methyl umbelliferone thiosemicarbazone and 3 acetyl umbelliferone thiosemicarbazone have been synthesized.The mechanism of the interaction between the complexes and calf thymus DNA has been studied by fluorescence method by using ethidium bromide EthBr as a fluorescence probe.The antitumor activities and the association constants of complexes were estimated.The results of the proposed fluorescence method is in agreement with those of the MTT method. |
| Keywords: | fluorescence screening test palladium complex antitumor activity mechanism of interaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |