伊贝母体细胞胚和器官发生几种大分子活性的比较研究

本文报道了伊贝母组织培养中体细胞胚发生和器官发生过程中的核酸、蛋白质含量及过氧化物酶活性动态的变化。共同特点是，培养初期几种大分子物质的含量均呈显著上升的趋势。说明两种途径均伴随着大分子物质的迅速合成。以后随着形态建成的进程，也都呈现相似的趋势。而ＲＮＡ和蛋白的合成总是先于ＤＮＡ的合成，说明这几种大分子的动态变化具有顺序性。其区别在于，体细胞胚胎发生过程几种大分子的活性高于器官发生过程，前者是一个

The Comparative Study of Several Macromolecules Activities Somatic Embryogenesis and Organogenesis of F.Pallidiflora Schrenk

In the paper,synthetic kinesis of nucleic acid and protein and peroxidase insomatic embryogenesis and organogenesis of F.Pallidiflora schrenk was comparativelystudied.Common features are:in the early stage of culture,the contents of severalmacromolecules markedly rise.This shows that either formation of embryonic cells,ordedifferentiation of organogenesis is always accompanied by rapid synthesis of macro-molecules.Later,they show a similar synthetic tendency in morphogenesis.Besides,RNA and protein synthesis are always in advance of DNA.This shows that the kineticchange of several macromolecules has a certain regularity.Their differences are:Theactivity of several macromolecules in the process of somatic embryogenesis is obviouslyhigher than that in the process of organogenesis.Therefore,the somatic embryogenesisis a more active physiologic and biochemical process.Besides,the firest peak curves ofRNA,DNA,protein and peroxidase in somatic embryogenesis appeared after 3,5,3,and 5 days respectively,but they appeared 5,10,5 and 10 days in organogenesis.Thisdifference reflects the variance of two pothways.It simultaneously illustrates thatmetabolic kinesis of macromolecules is always concerned with morphogenesis.