| TTC-脱氢酶还原法测定滇重楼细胞活力优化条件筛选 |
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| 引用本文： | 王跃华,刘益丽,马良良,林抗雪,代勇,徐世军,王晓蓉.TTC-脱氢酶还原法测定滇重楼细胞活力优化条件筛选[J].成都大学学报(自然科学版),2011,30(1):1-3. |
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| 作者姓名： | 王跃华 刘益丽 马良良 林抗雪 代勇 徐世军 王晓蓉 |
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| 作者单位： | 王跃华,刘益丽,马良良,林抗雪,WANG Yuehua,LIU Yili,MA Liangliang,LIN Kangxue(成都大学生物产业学院,四川成都,610106);代勇,徐世军,王晓蓉,DAI Yong,XU Shijun,WANG Xiaorong(成都恩威集团(投资)有限公司,四川成都,610041) |
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| 基金项目： | 国家"十二五"科技支撑计划资助项目 |
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| 摘 要： | 目的:采用2,3,5-氯化三苯基四氮唑(2,3,5-Triphenyl Tetrazolium Chloride,TTC)-脱氢酶还原法对超低温保存后的滇重楼地下茎进行细胞活力测定,旨在探索出一套更加简单、快速和准确的细胞活力测定方法.方法:选取TTC-脱氢酶还原法测定细胞活力过程中的TTC溶液浓度、pH值、反应温度、...
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| 关 键 词： | TTC法 正交设计 滇重楼 细胞活力 |
Optimization of TTC Method for Determination of Paris Polyphylla Var. Yunnanensis Cell Activity |
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| WANG Yuehua,LIU Yili,MA Liangliang,LIN Kangxue,DAI Yong,XU Shijun,WANG Xiaorong.Optimization of TTC Method for Determination of Paris Polyphylla Var. Yunnanensis Cell Activity[J].Journal of Chengdu University (Natural Science),2011,30(1):1-3. |
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| Authors: | WANG Yuehua LIU Yili MA Liangliang LIN Kangxue DAI Yong XU Shijun WANG Xiaorong |
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| Institution: | WANG Yuehua1,LIU Yili1,MA Liangliang1,LIN Kangxue1,DAI Yong2,XU Shijun2,WANG Xiaorong2(1.School of Bioindustry,Chengdu University,Chengdu 610106,China,2.Chengdu Enwei Group Co.,Chengdu 610041,China) |
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| Abstract: | To establish a more convenient,fast and accurate system for the determination of cell viability,TTC method was used to determine the underground stem's cell viability of Paris polyphylla var.yunnanensis after cryopreservation.TTC concentration,pH,staining temperature and time were chosen as four factors for orthogonal design.The relative survival rate of the cell was taken as viewing index and every factor was given four calculating values in order to optimize the determining conditions.The findings show th... |
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| Keywords: | TTC method orthogonal design Paris polyphylla var yunnanensis cell activity |
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