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| 乙肝病毒核酸疫苗诱导小鼠细胞免疫应答的研究 | |
| 引用本文： | 刘丹,谢晓娜,刘嵬,王智,郭晓强,苟小军.乙肝病毒核酸疫苗诱导小鼠细胞免疫应答的研究[J].成都大学学报(自然科学版),2003,22(2):15-17. |
| 作者姓名： | 刘丹 谢晓娜 刘嵬 王智 郭晓强 苟小军 |
| 作者单位： | 1. 长春生物制品研究所,长春,130062 2. 吉林大学分子酶学工程教育部重点实验室,长春,130021 3. 成都大学分子进化工程实验室,成都,610106 |
| 摘 要： | 研究利用核酸疫苗诱生乙型肝炎病毒 (HBV)表面抗原 (S)细胞免疫应答的作用 .免疫前于BALB/C小鼠股四头肌注射 75 %盐酸布比卡因 ,三天后同样部位注射包含HBsAg基因片段的重组质粒pcDNAHBs .采用3H -TdR掺入法测定免疫小鼠脾细胞增殖能力 ;XTT法测定其脾细胞分泌IL 2活性 .结果表明注射乙肝核酸疫苗可以使小鼠脾细胞增殖 ,小鼠脾细胞分泌上清中可检测到IL - 2活性 |
| 关 键 词： | 乙型肝炎表面抗原 核酸疫苗 细胞免疫 |
| 文章编号： | 1004-5422(2003)02-0015-03 |
Cell Immune Response Induced by Hepatitis B Virus DNA Vaccine in Mice |
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| LIU Dan ,XIE Xiaona ,LIU Wei ,WANG Zhi ,GUO Xiaoqiang ,GUO Xiaojun.Cell Immune Response Induced by Hepatitis B Virus DNA Vaccine in Mice[J].Journal of Chengdu University (Natural Science),2003,22(2):15-17. | |
| Authors: | LIU Dan XIE Xiaona LIU Wei WANG Zhi GUO Xiaoqiang GUO Xiaojun |
| Institution: | LIU Dan 1,XIE Xiaona 2,LIU Wei 2,WANG Zhi 2,GUO Xiaoqiang 3,GUO Xiaojun 3 |
| Abstract: | To investigate cell immune response in mice induced with HBsAg DNA vaccine. BALB/C mice were injected with Paracaine and were immuned with recombinant plasmid pcDNAHBs after three days. The proliferative ability of splenocyte were detected by 3H-TdR method and the activity of IL-2 were detected by XTT. The results demonstrated that the Hepatitis B DNA vaccine can induce cell immune response in mice. |
| Keywords: | Hepatitis B DNA vaccine cell immune |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |