| 川贝母培养物快速生产中的有效除菌研究 |
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| 引用本文： | 王跃华,张珏,江明殊,郭翠平,何诗红,王晓蓉,刘涛.川贝母培养物快速生产中的有效除菌研究[J].成都大学学报(自然科学版),2013(3):229-231. |
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| 作者姓名： | 王跃华 张珏 江明殊 郭翠平 何诗红 王晓蓉 刘涛 |
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| 作者单位： | [1]成都大学生物产业学院,四川成都610106 [2]四川师范大学生命科学学院,四川成都610101 [3]成都恩威集团（投资）有限公司,四川成都610041 |
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| 基金项目： | 成都市科技计划（12GGYB413SW-001）资助项目. |
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| 摘 要： | 在MS培养基中分别添加不同浓度的头孢噻肟钠对染菌川贝母组培物进行抗生素除菌培养,分析头孢噻肟钠对染菌川贝母组培物的除菌效果.实验表明,采用浓度为500 mg·L-1的头孢噻肟钠抗生素培养基连续进行2次除菌处理培养,对染菌的川贝母组培材料的除菌效果最好,其除菌率高达96.3%.
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| 关 键 词： | 川贝母 头孢噻肟钠 除菌 |
Study on Degerming Effects of Fritillaria Cirrhosa D.Don in Rapid Production |
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| WANG YuehuaI,ZHANG Jue,JIANG Mingshu,＇,GE HE ShihongI,WANG Xiaorong,LIU Tao.Study on Degerming Effects of Fritillaria Cirrhosa D.Don in Rapid Production[J].Journal of Chengdu University (Natural Science),2013(3):229-231. |
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| Authors: | WANG YuehuaI ZHANG Jue JIANG Mingshu ＇ GE HE ShihongI WANG Xiaorong LIU Tao |
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| Institution: | 1 (1 .School of Biological Industry, Chengdu University, Chengdu 610106, China; 2.College of Life Science, Sichnan Normal University, Chengdu 610101, China; 3.Chengdu Enwei Group Co., Ltd., Chengdu 610041, China) |
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| Abstract: | To study the degerming effects of bacterial contamination of Fritillaria cirrhosa D. Don with dif- ferent concentrations of cefotaxime sodium, we degerm the bacterial contamination of Fritillaria cirrhosa D. Don by adding different concentrations of cefotaxime sodium in the MS medium. The experimental results show that after degerming two times continuously with cefotaxime sodium at the concentration of 500 mg· L-1, there is the best degermation and the degerming rate is 96.3 %. |
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| Keywords: | Fritillaria cirrhosa D Don cefotaxime sodium degermation |
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