| RP-HPLC法测定复方丹参胶囊中丹参素的含量 |
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| 引用本文： | 邹亮,罗亚玲,赵钢,杨敬东.RP-HPLC法测定复方丹参胶囊中丹参素的含量[J].成都大学学报(自然科学版),2006,25(3):207-209. |
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| 作者姓名： | 邹亮 罗亚玲 赵钢 杨敬东 |
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| 作者单位： | 成都大学,生物工程系,四川,成都,610106;成都中医药大学,四川,成都,610075;四川省药品检验所,四川,成都,610070;成都大学,生物工程系,四川,成都,610106 |
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| 摘 要： | 为测定复方丹参胶囊中丹参素含量,采用反相高效液相色谱法,D ikm a HPLC column色谱柱(5μm,200×4.6 mm),以乙腈—水—冰醋酸(5∶160∶1)为流动相,流速1.0 mL/m in,检测波长280nm,柱温30℃测定.RP-HPLC测定的线性范围为0.28~1.40μg,相关系数为r=0.9998;胶囊中丹参素的平均回收率为100.17%,RSD=0.78%(n=6),精密度良好(RSD=0.64%).该法操作简便、快速、准确,适用于复方丹参胶囊中丹参素的含量测定.
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| 关 键 词： | 复方丹参胶囊 反相高效液相色谱法 丹参素 含量测定 |
| 文章编号： | 1004-5422（2006）03-0207-03 |
| 收稿时间： | 2006-04-26 |
| 修稿时间： | 2006-04-26 |
Determination of Danshensu in Compound Danshen Capsules by RP-HPLC |
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| ZOU Liang,LUO Yaling,ZHAO Gang,YANG Jingdong.Determination of Danshensu in Compound Danshen Capsules by RP-HPLC[J].Journal of Chengdu University (Natural Science),2006,25(3):207-209. |
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| Authors: | ZOU Liang LUO Yaling ZHAO Gang YANG Jingdong |
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| Abstract: | To determine the content of Danshensu in Compound Danshen Capsules,RP-HPLC method is used.The samples are separated on Dikma HPLC column with the mobile phrase of acetonitrile-water-glacial acetic(5∶160∶1).The flow-rate is 1.0mL:min1 and the wavelength is 280nm with column temperature of 30℃.The linear calibration curves are obtained at the range of 0.28~1.40μg(r=0.9998).The average recovery of Danshensu is 100.17%(RSD=0.78%,n=6) in the capsules.The method is precise(RSD=0.64%).The method is simple,quick and accurate,and proves suitable for determining the content of Danshensu in Compound Danshen Capsules. |
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| Keywords: | Compound Danshen Capsules RP-HPLC Danshensu content determination |
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