| 分光光度法测定Fenton反应产生的羟基自由基 |
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| 引用本文： | 颜军,苟小军,邹全付,纪小明,崔秀英,兰海蓉,李雪.分光光度法测定Fenton反应产生的羟基自由基[J].成都大学学报(自然科学版),2009,28(2):91-93,103. |
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| 作者姓名： | 颜军 苟小军 邹全付 纪小明 崔秀英 兰海蓉 李雪 |
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| 作者单位： | 成都大学中药化学实验室,四川,成都,610106 |
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| 基金项目： | 四川省中医药管理局科技专项基金资助项目 |
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| 摘 要： | 基于水杨酸可以捕获羟基自由基（·OH）生成2，3-二羟基苯甲酸和2，5-二羟基苯甲酸，在波长510nm处有最大吸收的原理，建立一种用分光光度法检测Fenton反应产生的羟基自由基（·OH）的方法．通过对反应体系影响条件的优化，筛选出最佳反应物配比和最佳实验条件，并对其稳定性进行检测．在此基础上。测定抗氧化剂对羟基自由基（·OH）的清除率．结果表明：该体系反应灵敏、稳定性高，可作为抗氧化剂的筛选方法之一.
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| 关 键 词： | Fenton反应 羟基自由基 分光光度法 水杨酸 |
Determination of Hydroxyl Radical Generating from Fenton Reaction by Spectrophotometry |
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| YAN Jun,GOU Xiaojun,ZOU Quanfu,JI Xiaoming,CUI Xiuying,LAN Hairong,LI Xue.Determination of Hydroxyl Radical Generating from Fenton Reaction by Spectrophotometry[J].Journal of Chengdu University (Natural Science),2009,28(2):91-93,103. |
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| Authors: | YAN Jun GOU Xiaojun ZOU Quanfu JI Xiaoming CUI Xiuying LAN Hairong LI Xue |
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| Institution: | Chemistry Laboratory of Traditional Chinese Medicine;Chengdu University;Chengdu 610106;China |
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| Abstract: | To produce 2,3-dihydroxy benzoic and 2,5-dihydroxy benzoic,salicylic acid was used to catch hydroxyl radical(·OH) by spectrophotometer determining hydroxyl radical produced by Fenton reaction.The reactants have an absorption maximum at 510 nm.Reaction system was optimized to screen out the best reactant match and optimal experimental conditions and its stability was tested.Based on the above,clearance rate of antioxidant on hydroxyl radical was measured.The findings show that this system has sensitive reaction,high stability and can be used to filter antioxidant. |
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| Keywords: | Fenton reaction hydroxyl radical spectrophotometry salicylic acid |
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