| Behavior of MC3T3-E1 Osteoblast Cultured on Chitosan Modified with Polyvinylpyrrolidone |
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| 作者姓名： | 奚静 高媛 孔丽君 公衍道 赵南明 张秀芳 |
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| 作者单位： | [1]Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China |
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| 基金项目： | Supported by the National High-Tech Research and Development（863） Program of China （Nos. 2001AA222053, 2002AA212051,and 2002AA207006） and the National Natural ScienceFoundation of China （No. 30270753） |
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| 摘 要： | The physical and chemical properties of four kinds of modified chitosan materials made by blending chitosan with polyvinylpyrrolidone （PVP） were investigated. All four of these modified chitosan materials were hydrophilic with water contact angles ranging from 59°to 69°. Fourier transform-infrared spectra of the modified materials showed a new band at 1288 cm^-1, implying formation of a surface physical interpenetrating network structure. Enzyme linked immunosorbent assay results indicated that much less fibronectin was adsorbed on the modified materials than on only chitosan. The viability of MC3T3-E1 osteoblasts cultured on the materials was assessed by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl- 2H-tetrazolium bromide assay. The results show that adding PVP10000 into the chitosan promotes adhesion of MC3T3-E1 osteoblasts on the modified materials, but has no effect on cell growth and proliferation; while adding PVP40000 reduces cell adhesion, growth, and proliferation. The results suggest that the increased hydrophilicity of the material surface does not always improve its biocompatibility, which will influence the selection and design of biomaterials.
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| 关 键 词： | MC3T3-E1 造骨细胞 壳聚糖 聚乙烯吡咯烷酮 细胞活性 |
| 收稿时间： | 2004-11-20 |
| 修稿时间： | 2004-11-202005-01-07 |
Behavior of MC3T3-E1 Osteoblast Cultured on Chitosan Modified with Polyvinylpyrrolidone |
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| Jing Xi,
&#x;, Yuan Gao, «, Lijun Kong, í , Yandao Gong,
&#x;, Nanming Zhao,
&#x;,Xiufang Zhang, .Behavior of MC3T3-E1 Osteoblast Cultured on Chitosan Modified with Polyvinylpyrrolidone[J].Tsinghua Science and Technology,2005,10(4):439-444. |
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| Authors: | Jing Xi
&#x; Yuan Gao « Lijun Kong í Yandao Gong
&#x; Nanming Zhao
&#x; Xiufang Zhang |
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| Institution: | Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | The physical and chemical properties of four kinds of modified chitosan materials made by blending chitosan with polyvinylpyrrolidone (PVP) were investigated. All four of these modified chitosan materials were hydrophilic with water contact angles ranging from 59° to 69°. Fourier transform-infrared spectra of the modified materials showed a new band at 1288 cm?1, implying formation of a surface physical interpenetrating network structure. Enzyme linked immunosorbent assay results indicated that much less fibronectin was adsorbed on the modified materials than on only chitosan. The viability of MC3T3-E1 osteoblasts cultured on the materials was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl- 2H-tetrazolium bromide assay. The results show that adding PVP10000 into the chitosan promotes adhesion of MC3T3-E1 osteoblasts on the modified materials, but has no effect on cell growth and proliferation; while adding PVP40000 reduces cell adhesion, growth, and proliferation. The results suggest that the increased hydrophilicity of the material surface does not always improve its biocompatibility, which will influence the selection and design of biomaterials. |
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| Keywords: | chitosan polyvinylpyrrolidone (PVP) cell viability fibronectin |
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