| Electroporation Transfection as an Effective Tool to Trace Transplanted NSCs in Adult Central Nervous System |
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| 作者姓名： | 周畅 温哲鈃 王志萍 郭行 史冬梅 左焕琮 谢佐平 |
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| 作者单位： | StateKeyLaboratoryofBiomembraneandMembraneBiotechnology,DepartmentofBiologicalSciencesandBiotechnology,TsinghuaUniversity,Beijing100084,China |
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| 基金项目： | the National Natural Science Foundation of China (No. 30070245) |
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| 摘 要： | Neural stem cells, which are clonogenic cells with self-renewal and multilineage differentiation properties, are currently considered as powerful candidates for cell replacement therapy in neurodegenerative disorders such as Parkinson‘s disease. A key issue is whether stem cells can survive, migrate and differentiate following transplantation into the adult central nervous system. This research shows that enhanced green fluorescent protein (EGFP) plasmid electreporation transfected neural stem cells can functionally differentiate in vitro and that most of the EGFP-positive cells can survive and migrate towards the damaged areas when transplanted into the brain of a Parkinson‘s disease model rat. The results suggest an effective and maneuverable tracing tool to detect whether transplanted neural stem and progenitor cells function in the adult brain in vivo.
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| 关 键 词： | 电穿孔转移感染 NSCs 中枢神经系统 成年人 神经干细胞 帕金森氏病 |
Electroporation Transfection as an Effective Tool to Trace Transplanted NSCs in Adult Central Nervous System |
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| ZHOU Chang,WEN Zhexing ,WANG Zhiping,GUO Xing,SHI Dongmei.Electroporation Transfection as an Effective Tool to Trace Transplanted NSCs in Adult Central Nervous System[J].Tsinghua Science and Technology,2004,9(1):81-87. |
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| Authors: | ZHOU Chang WEN Zhexing WANG Zhiping GUO Xing SHI Dongmei |
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| Abstract: | |
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| Keywords: | neural stem cell electroporation transfection enhanced green fluorescent protein transplantation migrate |
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