| Construction of Yeast Vectors with Resistance to Geneticin |
| |
| 作者姓名： | 林会兰 张广 周全 陈国强 |
| |
| 作者单位： | LIN Huilan，ZHANG Guang，ZHOU Quan CHEN Guoqiang Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China |
| |
| 摘 要： | IntroductionYeast Saccharomyces cerevisiae (S.cerevisiae) hasbeen widely used in molecular biology as well asinthe industrial production.Its genetic backgroundhas been well studied and a series of cloning andexpression vectors have been constructed for DNAtransformation into yeast1 ] .The selection markersof these conventional vectors are usually H IS3 ,TRP1 ,LEU2 ,and URA3 1 ,2 ] . These selectionmarkers are responsible for the biosynthesis ofsome amino acids and consequently the hos…
|
Construction of Yeast Vectors with Resistance to Geneticin |
| |
| LIN Huilan,ZHANG Guang,ZHOU Quan CHEN Guoqiang.Construction of Yeast Vectors with Resistance to Geneticin[J].Tsinghua Science and Technology,2002,7(4). |
| |
| Authors: | LIN Huilan ZHANG Guang ZHOU Quan CHEN Guoqiang |
| |
| Institution: | LIN Huilan,ZHANG Guang,ZHOU Quan CHEN Guoqiang Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China |
| |
| Abstract: | Two Escherichia coli Saccharomyces cerevisiae shuttle vectors containing a resistance marker to geneticin (G418) are constructed. Both vectors contain a kanamycin resistant marker (KanMX4) module coding aminoglycoside 3' phosphotransferase (APH) that renders E. coli resistant to kanamycin and S. cerevisiae to geneticin. These vectors overcome the shortage of the conventional yeast vectors bearing HIS3, TRP1, LEU2, and URA3 modules as selection markers, which require hosts to be auxotrophic. Green fluorescent protein (GFP) is used as the reporter to examine the functions of the vectors. The vectors are powerful tools for the convenient cloning and controlled expression of genes in most S. cerevisiae strains. |
| |
| Keywords: | geneticin (G418) shuttle vector green fluorescent protein (GFP) Escherichia coli (E coli) Saccharomyces cerevisiae |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
