| Formation of Polyhydroxyalkanoate Blends by Pseudomonas pseudoalcaligenes M1-2 from Various Carbon Sources |
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| 作者姓名： | 吴琼 张广 田卫东 孙素琴 张日清 陈国强 |
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| 作者单位： | WU Qiong，ZHANG Guang，TIAN Weidong，SUN Suqin ，ZHANG Riqing，CHEN Guoqiang Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing\ 100084 Department of Chemistry，Tsinghua University，Beijing\ 100084 |
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| 基金项目： | the Trans-Century Training Pro gramme Foundation for Talent by the Ministry of Education of China and the Major Research |
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| 摘 要： | ＩｎｔｒｏｄｕｃｔｉｏｎＰｏｌｙｈｙｄｒｏｘｙａｌｋａｎｏａｔｅｓ (ＰＨＡｓ)ａｒｅａｆａｍｉｌｙｏｆｉｎｔｒａｃｅｌｌｕｌａｒｂｉｏｐｏｌｙｍｅｒｓｓｙｎｔｈｅｓｉｚｅｄｂｙｍａｎｙｂａｃｔｅｒｉａａｓｉｎｔｒａｃｅｌｌｕｌａｒｃａｒｂｏｎａｎｄｅｎｅｒｇｙｓｔｏｒａｇｅｃｏｍｐｏｕｎｄｓ1] .ＰＨＡｓｈａｖｅａｗｉｄｅｒａｎｇｅｏｆｐｈｙｓｉｃａｌｐｒｏｐｅｒｔｉｅｓｒａｎｇｉｎｇｆｒｏｍｂｒｉｔｔｌｅｔｏｆｌｅｘｉｂｌｅｔｏｅｌａｓｔｉｃ ,ｄｅｐｅｎｄｉｎｇｏｎｔｈｅｉｒｍｏｎｏｍｅｒｓｔｒ…
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Formation of Polyhydroxyalkanoate Blends by Pseudomonas pseudoalcaligenes M1-2 from Various Carbon Sources |
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| WU Qiong,ZHANG Guang,TIAN Weidong,SUN Suqin,ZHANG Riqing,CHEN Guoqiang.Formation of Polyhydroxyalkanoate Blends by Pseudomonas pseudoalcaligenes M1-2 from Various Carbon Sources[J].Tsinghua Science and Technology,1999,4(3). |
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| Authors: | WU Qiong ZHANG Guang TIAN Weidong SUN Suqin ZHANG Riqing CHEN Guoqiang |
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| Abstract: | Pseudomonas pseudoalcaligenes strain M1-2 isolated from oil-contaminated soil collected from an oilfield in northern China was found to be able to synthesize a blend of polyhydroxyalkanoates (PHAs) containing monomers of 3-hydroxybutyrate (C4), 3-hydroxyvalerate (C5), 3-hydroxyheptanoate (C7), 3-hydroxyoctanoate (C8), 3-hydroxynonanoate (C9), 3-hydroxydecanoate (C10) and 3-hydroxydodecanoate (C12) from various carbon sources.The hydroxyalkanoate (HA) monomer composition varied both quantitatively and qualitatively, depending on the carbon sources used.The presence of octanoate in substrates of myristic acid or tridecanoate promoted the synthesis of HB monomer in the blend.Concentration of octanoate was also found to significantly affect the PHB content in the blend.A PHA biosynthesis pathway in Pseudomonas pseudoalcaligens M1-2 was proposed. |
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| Keywords: | polyhydroxyalkanoate PHA polyhydroxybutyrate PHB Pseudomonas pseudoalcaligenes |
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