| Direct Promotion of Collagen Calcification by Alkaline Phosphatase |
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| 作者姓名： | 王术 公衍道 张秀芳 赵南明 |
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| 作者单位： | WANG Shu，GONG Yandao，ZHANG Xiufang，ZHAO Nanming State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing l00084，China [ |
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| 基金项目： | the National Natural Science Foundation
of China! (No.5 94932 0 8) |
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| 摘 要： | IntroductionAlkalinephosphatase(AP)hasbeenthoughttobeimportantintheprocessofbiocalcification1].APwasthefirstenzymeassociatedwithcalcificationactivityintissuesandremainsasthebestcharacterizedenzymeinthecalcificationprocess.Yet,importantquestionsabouttheroleofthisproteininbiocalcificationremainunanswered,anditcontinuestodrawfocusedattentionincalcifiedtissueinvestigations2].APpromoteshydrolysisofphosphatecontainingsubstrates1],producesorthophosphateandcausesariseininorganicphosphateandtherem…
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Direct Promotion of Collagen Calcification by Alkaline Phosphatase |
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| WANG Shu,GONG Yandao,ZHANG Xiufang,ZHAO Nanming.Direct Promotion of Collagen Calcification by Alkaline Phosphatase[J].Tsinghua Science and Technology,2000,5(4). |
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| Authors: | WANG Shu GONG Yandao ZHANG Xiufang ZHAO Nanming |
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| Abstract: | WTFZ] Alkaline phosphatase promotes hydrolysis of phosphate containing substrates, causes a rise in inorganic phosphate and, therefore, enhances calcification of biological tissues. In this work, the calcification of collagen in a model serum was used as a model of collagenous tissue biomaterials to study the possible calcification promotion mechanism of alkaline phosphatase. In the enzyme concentration range of 0.10.5mg/mL, the enzyme shows a direct calcification promoting effect which is independent of the hydrolysis of its phosphate containing substrates but proportional to the enzyme concentration. Potassium pyrophosphate somewhat inhibits the calcification promotion. |
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| Keywords: | ZK(]alkaline phosphatase calcification promotion effect collagenl |
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