| Induction of Apoptosis in Protoplasts and Suspension Cultures of Plant Cells |
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| 作者姓名： | 颜长辉 周军 陈浩明 戴尧仁 |
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| 作者单位： | YAN Changhui，ZHOU Jun，CHEN Haoming，DAI Yaoren Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China [ |
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| 摘 要： | IntroductionApoptosisisacellularautodestructionprocessfortheactiveeliminationofunwantedcells1].Apoptosishasbeenobservedinmanyanimaltissuesandwasfoundtoplayavitalroleinnormaldevelopment,maintenanceofhomeostasisandpathologicalprocessesassociatedwithvariousdiseases2,3].Thereismoreandmoreevidenceshowingthatapoptosisalsooccursinhigherplants4].However,comparedwiththeresearchinanimals,thestudyofapoptosisinplantsisstillinitsinitialstage.Ourstudyinvestigatestheapoptosis-inducingeffectsofsomeabiotic…
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Induction of Apoptosis in Protoplasts and Suspension Cultures of Plant Cells |
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| YAN Changhui,ZHOU Jun,CHEN Haoming,DAI Yaoren.Induction of Apoptosis in Protoplasts and Suspension Cultures of Plant Cells[J].Tsinghua Science and Technology,2000,5(4). |
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| Authors: | YAN Changhui ZHOU Jun CHEN Haoming DAI Yaoren |
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| Abstract: | Many studies have showed that apoptosis exists in plants. Our study shows that (1) menadione(VK3) induces apoptosis in suspension cultures of carrot cells; (2) heat shock induces apoptosis in suspension cultures of tobacco cells; and (3) ethrel induces apoptosis in carrot protoplasts. Some important indications of apoptosis were observed, including DNA laddering, TUNEL-positive reaction, condensation and degradation of nuclei. |
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| Keywords: | apoptosis protoplasts suspension culture carrot tobacco menadione ethrel heat shock |
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