| 酵母双杂交系统的研究进展 |
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| 引用本文： | 吴娟,钱凯,杨泽峰.酵母双杂交系统的研究进展[J].安庆师范学院学报(自然科学版),2005,11(2):59-63. |
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| 作者姓名： | 吴娟 钱凯 杨泽峰 |
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| 作者单位： | 安庆师范学院,生命科学系,安徽,安庆,246011;扬州大学,生物科学与技术学院,江苏,扬州,225009;扬州大学,农学院,江苏,扬州,225009 |
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| 摘 要： | 酵母双杂交系统自创建以来,已成为研究蛋白质相互作用的重要手段,揭示了大量未知蛋白质之间的相互作用。随着该系统的广泛应用,近年来又发展了三杂交系统、单杂交系统、逆向双杂交系统、SOS富集系统等。酵母双杂交及其衍生系统已经成功地运用于蛋白质之间,蛋白质与DNA、RNA、配体之间相互作用的研究。
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| 关 键 词： | 酵母双杂交系统 蛋白质间相互作用 三杂交系统、AD DNA-BD |
| 文章编号： | 1007-4260(2005)02-0059-05 |
The Theory, Application and Development of Yeast Two-hybrid System |
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| WU Juan,QIAN Kai,YANG Ze-feng.The Theory, Application and Development of Yeast Two-hybrid System[J].Journal of Anqing Teachers College(Natural Science Edition),2005,11(2):59-63. |
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| Authors: | WU Juan QIAN Kai YANG Ze-feng |
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| Abstract: | The yeast two-hybrid system has become an important method to research the protein-protein interactions. Since its creation, many protein-protein interactions between unknown proteins have been revealed with the using of it. With its wide applications, many new research techniques have been developed in recent years, such as three-hybrid system, one-hybrid system, reverse two-hybrid system and SOS recruitment systems. The yeast two-hybrid system and its derived system have enabled us to study the interactions between proteins and DNA, RNA and small organic ligands. |
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| Keywords: | yeast two-hybrid system protein-protein interactions three-hybrid system AD DNA-BD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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