红土沉香的化学成分及其抗炎活性研究

综合运用多种柱色谱技术从越南红土沉香乙醇提取物中分离得到13个化合物，经过现代波谱学技术鉴定其结构为：7-甲氧基-2-2-(4-甲氧基苯基)乙基]色酮(1),6-甲氧基-2-(2-苯乙基)色酮(2),6,7-二甲氧基-2-(2-苯乙基)色酮(3),6-甲氧基-2-2-(4-甲氧基苯基)乙基]色酮(4),6-甲氧基-2-2-(3-甲氧基苯基)乙基]色酮(5),5-羟基-6-甲氧基-2-2-(4-甲氧基苯基)乙基]色酮(6),6,8-二羟基-2-(2-苯乙基)色酮(7)，(1β，3α，4aβ，5β，8aα)-4a,5-dimethyl-3-(prop-1-en-2-yl)octahydronaphthalene-1,8a(1H)-diol(8)，rel-(4S,5R,7R,8R,11R)-eremophil-9-en-12,8-olide(9)，cyclodebneyol(10)，柯拉斯那酸甲酯(11)，白木香醇(12)和4-表-15-羟基菖蒲螺烯酮(13).其中，化合物1为新天然产物，所有化合物均为首次从红土沉香中分离得到.以上化合物的抗炎活性测试结果表明，化合物3、8和13具有一定的抗炎活性，IC50值分别为81.23 ± 2.83、39.55 ± 3.75和72.63 ± 2.87 μmol·L－1.

Chemical constituents from agarwood “Hong-Tu” and their anti-inflammatory activity

Thirteen compounds were isolated from the ethanol extract of agarwood “Hong-Tu” by using various column chromatographic techniques. Their structures were elucidated by spectroscopic data analysis as 7-methoxy-2-2-(4-methoxyphenyl)ethyl]chromone (1)， 6-methoxy-2-(2-phenylethyl)chromone (2)， 6,7-dimethoxy-2-(2-phenylethyl)chromone (3)， 6-methoxy-2-2-(4-methoxyphenyl)ethyl]chromone (4)， 6-methoxy-2-2-(3-methoxyphenyl)ethyl]chromone (5)， 5-hydroxy-6-methoxy-2-2-(4-methoxyphenyl) ethyl]chromone (6)， 6,8-dihydroxy-2-(2-phenylethyl)chromone (7)，(1β，3α，4aβ，5β，8aα)-4a,5-dimethyl-3-(prop-1-en-2-yl)octahydronaphthalene-1,8a(1H)-diol (8)， rel-(4S,5R,7R,8R,11R)-eremophil-9-en-12,8-olide (9)， cyclodebneyol (10)， methyl crassicid (11)， baimuxinol (12) and 4-epi-15-hydroxyacorenone (13). Compound 1 was a new natural product. All of them were firstly isolated from agarwood “Hong-Tu”. In vitro anti-inflammatory assessment showed that compounds 3， 8 and 13 exhibited anti-inflammatory activities with IC50 values of 81.23 ± 2.83， 39.55 ± 3.75 and 72.63 ± 2.87 μmol·L－1， respectively.