柱层析循环联合法提取双刺参胶囊中的总皂苷及多糖

探索柱层析循环法联合提取双刺参胶囊中总皂苷及多糖的工艺条件，并对总皂苷体外活性进行研究.以提取液中总皂苷及多糖提取率为指标，通过对提取溶剂、吸涨率、浸泡平衡时间、洗脱流速等因素的考察确定提取的最佳工艺.结果显示，总皂苷及多糖提取溶剂分别为40%乙醇溶液和纯水、吸涨率分别为3.8 mL·g－1和3.6 mL·g－1、浸泡平衡时间均为2 h、接样速率为0.4 mL·min－1.在此条件下，总皂苷及多糖经过2轮连续提取，提取率均在95%以上，浸膏中总皂苷及多糖的含量分别为18.05%和48.37%.体外抗氧化试验表明，双刺参提取物具有一定的抗氧化能力.利用柱层析循环联合法提取总皂苷及多糖，提取温度低、乙醇用量少、能源消耗低、方法简单，为双刺参胶囊的进一步研究奠定了基础.

Circulating column chromatographic extraction of total saponin and polysaccharide of Shuangcishen Capsule

The process conditions of extracting total saponins and polysaccharides from Shuangcishen Capsules by column chromatography cycle were explored，and the activity of total saponins in vitro were also studied. Based on the extraction rate of total saponins and polysaccharides in the extract， the optimal extraction process was determined by investigating factors such as extraction solvent， suction rate， soaking equilibrium time and elution flow rate. The results showed that the total saponins and polysaccharide extraction solvent were 40% ethanol solution and pure water， respectively. The absorption rates were 3.8 mL·g－1 and 3.6 mL·g－1， respectively，with the soaking equilibrium time of 2 h and the sampling rate of 0.4 mL·min－1. Under these conditions， the total saponins and polysaccharides were continuously extracted in 2 rounds， and the extraction rates were all above 95%. The extracts were mixed and the total saponins and polysaccharides were separated by alcohol precipitation. The contents of total saponins and polysaccharides in the extract were 18.05% and 48.37%， respectively. The crude polysaccharide was purified by two times of water-soluble alcohol precipitation， with the content increasing to 69.91%.The results of the antioxidant test showed that the extract of Shuangcishen had certain antioxidant activity. The total saponins and polysaccharides were extracted by column chromatography cycle combined with low extraction temperature， low ethanol consumption， low energy consumption and simple operation， which offered a foundation for further research of Shuangcishen Capsules.