| 邻苯二酚与脱氧核糖核酸相互作用的研究 |
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| 引用本文： | 张建明,宋远志,石德清.邻苯二酚与脱氧核糖核酸相互作用的研究[J].华中师范大学学报(自然科学版),2005,39(3):362-364,369. |
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| 作者姓名： | 张建明 宋远志 石德清 |
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| 作者单位： | 华中师范大学化学学院,武汉,430070;淮阴师范学院化学系,江苏淮安,223300 |
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| 基金项目： | 国家青年自然科学基金资助项目（20302002）;江苏省教育厅自然科学基金资助项目（04KJD150038）. |
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| 摘 要： | 用循环伏安法、荧光光谱法、紫外可见光谱法、量子化学方法研究了pH为7．42的磷酸盐缓冲溶液中邻苯二酚与脱氧核糖核酸（DNA）的相互作用，在DNA开链过程中，邻苯二酚与脱氧核糖核酸作用后的荧光光谱曲线、紫外光谱曲线的吸收峰改变较大，循环伏安法峰氧化峰正向移动，电流增大，量化计算表明邻苯二酚位于磷酸基团近侧，邻苯二酚与脱氧核糖核酸主要以静电方式结合。
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| 关 键 词： | 邻苯二酚 脱氧核糖核酸(DNA) 相互作用 |
| 文章编号： | 1000-1190（2005）03-0362-03 |
| 收稿时间： | 2004-11-12 |
| 修稿时间： | 2004-11-12 |
Study on the interaction between catechol and deoxgribonucleic acid |
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| ZHANG Jian-ming,SONG Yuan-zhi,SHI De-qing.Study on the interaction between catechol and deoxgribonucleic acid[J].Journal of Central China Normal University(Natural Sciences),2005,39(3):362-364,369. |
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| Authors: | ZHANG Jian-ming SONG Yuan-zhi SHI De-qing |
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| Institution: | 1. College of Chemistry, Central China Normal University, Wuhan 430070; Department of Chemistry, Huai'an Teachers' College, Huai'an, Jiangsu 223300 |
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| Abstract: | For study on the interaction between catechol and deoxgribonucleic acid, ultraviolet-visble spectrophotometry, fluorescence spectrophotometry, cyclic voltammetry and quantum chemistry were used to study the interaction between catechols and deoxgribonucleic acid. In sodium phosphate buffer solution pH 7.42, the peaks and intension of absorption and fluorescence spectra change markedly, the peak of cyclic voltammetry shift to positive and its peak current increase. The theoretical calculation indicates that the catechols close to the phosphate groups in DNA. The electrostatic attraction between catechols and deoxgribonucleic acid are the major modes for the interaction. |
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| Keywords: | catechol deoxgribonucleic acid interaction |
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