日本血吸虫体壁抗原基因的筛选与克隆

为筛选并克隆新的日本血吸虫(Schistosoma japonicum,Sj)候选抗原基因,以日本血吸虫成虫总DNA为模板,通过生物信息学以及分子生物学手段从日本血吸虫全基因组中筛选目的基因.根据所筛选到的目的基因的核酸序列设计合成引物,用PCR法扩增目的基因,将其克隆入pBS-T载体后转入到大肠杆菌DH5a菌株,筛选阳性克隆.通过对阳性菌落质粒进行PcR予以证实.最终成功筛选并克隆到日本血吸虫体壁候选抗原基因.

Sequencing and cloning the integument gene encoding antigen of Schistosoma japonicum

Screening the target gene from the genome DN A of S. j aponicum using the audit S. japonicum DNA as template by bioinformatics and Molecular biology metholds. The designed primers according to the screening results were used to amplify the specific fragment by polymerase chain reaction (PCR). The Product of PCR carried into pBS-T vector was cloned into to the E. coli DH5a. The inserts of the positive clones were identified by plasmid PCR. Finally, New candidate genes encoding the integument antigens for vaccine against S. J aponicum have been successfully screened out, providing a foundation for further study of the target gene expressing in different gene expression system. Further study in the immunodiagnosis and immunotherapy against S. J aponicum can be conducted.