| 油菜薄层细胞培养中不同光质对愈伤组织诱导和增殖的效应 |
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| 引用本文： | 周吉源,赵洁,杨和平,程井辰.油菜薄层细胞培养中不同光质对愈伤组织诱导和增殖的效应[J].华中师范大学学报(自然科学版),1992,31(1):0-0. |
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| 作者姓名： | 周吉源 赵洁 杨和平 程井辰 |
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| 作者单位： | 华中师范大学生物系
(周吉源，赵洁，杨和平)，华中师范大学生物系(程井辰) |
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| 摘 要： | 本实验以油菜(Brassica napus L.)花序轴薄层细胞为外植体,基本培养基为MS,附加不同浓度和比例的NAA和6-BA组合。接种后,选取白光、植物生长灯、红光、蓝光四种不同光质的光进行光照,以黑暗为对照,置恒温培养柜中培养,探讨不同光质对愈伤组织诱导和增殖的效应。结果表明:在相同或不同的培养基上,不同光质对愈伤组织的诱导效应都不同,蓝光,红光对油菜愈伤组织诱导具有明显的促进作用。在相同培养基中,不同光质处理对愈伤组织的增殖效应也不同,蓝光、红光对愈伤组织增殖的促进作用也是明显的。
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| 关 键 词： | 光质效应 薄层细胞培养 愈伤组织 油菜 |
| 收稿时间： | 1992-01-25 |
EFFECT OF LIGHT QUALITY ON INDUCTION AND REPRODUCTION OF CALLUS IN THIN CELL LAYERS CULTURE OF BRASSICA NAPUS L. |
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| Zhou Jiyuan Zhao Jie Yang Heping Cheng Jinchen.EFFECT OF LIGHT QUALITY ON INDUCTION AND REPRODUCTION OF CALLUS IN THIN CELL LAYERS CULTURE OF BRASSICA NAPUS L.[J].Journal of Central China Normal University(Natural Sciences),1992,31(1):0-0. |
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| Authors: | Zhou Jiyuan Zhao Jie Yang Heping Cheng Jinchen |
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| Abstract: | This paper reports the effect of light quality on induction and reproduction of callus in thin cell layers cultures of Brassica napus L.. Explants were obtained by excised thin cell layers(four to nine layers of epidermal and subepidermal)from raches of Brassica napus L.. The explants cultured on MS' mediums with addition of NAA and 6-BA and irradiated under lights with different wavebands. The effect of light quality on induction and reproduction of callus in thin cell layers culture were observed. Blue and red light has a considerable effct on induction and reproduction of calli, while those of light in other wavebands are not apparent. |
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| Keywords: | effect of light quality thin cell layers culture callus Brassica napus L |
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