| TaNHX2基因植物表达载体的构建及在烟草中的表达 |
| |
| 引用本文： | 原江锋,杨建雄,俞嘉宁,王丽娟,张志琪,王喆之.TaNHX2基因植物表达载体的构建及在烟草中的表达[J].陕西师范大学学报,2007,35(3):78-82. |
| |
| 作者姓名： | 原江锋 杨建雄 俞嘉宁 王丽娟 张志琪 王喆之 |
| |
| 作者单位： | 陕西师范大学药用植物资源与天然药物化学教育部重点实验室，陕西师范大学药用植物资源与天然药物化学教育部重点实验室，陕西师范大学生命科学学院，宁夏大学生物学系，陕西师范大学药用植物资源与天然药物化学教育部重点实验室，陕西师范大学药用植物资源与天然药物化学教育部重点实验室 陕西西安710062，宁夏银川750021 |
| |
| 摘 要： | 将TaNHX2基因重组于质粒pBIN438的CaMV 35S启动子下游,构建含TaNHX2基因的植物双元表达载体pBIN438-TaNHX2.采用农杆菌介导转化烟草(Nicotiana tobacum L.),获得含TaNHX2的转基因烟草植株.经PCR、RT-PCR分析表明,TaNHX2基因已整合到烟草中,并且得到表达.耐盐分析表明,外源基因TaNHX2提高了转基因植株的耐盐性.
|
| 关 键 词： | 载体构建 烟草 基因表达 抗逆性 |
| 文章编号： | 1672-4291（2007）03-0078-05 |
| 修稿时间： | 2006-06-15 |
Studies on construction of TaNHX2 gene plant expression vector and Agrobacterium tumefaciens-mediated genetic transformation of Nicotiana tobacum L. |
| |
| YUAN Jiang-feng,YANG Jian-xiong,YU Jia-ning,WANG Li-juan,ZHANG Zhi-qi,WANG Zhe-zhi.Studies on construction of TaNHX2 gene plant expression vector and Agrobacterium tumefaciens-mediated genetic transformation of Nicotiana tobacum L.[J].Journal of Shaanxi Normal University: Nat Sci Ed,2007,35(3):78-82. |
| |
| Authors: | YUAN Jiang-feng YANG Jian-xiong YU Jia-ning WANG Li-juan ZHANG Zhi-qi WANG Zhe-zhi |
| |
| Abstract: | The TaNHX2 gene was constructed in plant bivalent expression vector pBIN438 to obtain a plant expression vector pBIN438-TaNHX2,in which the TaNHX2 gene was constructed at downstream CaMV 35S promoter.Based on the results mentioned above,the vector pBIN438-TaNHX2 was introduced into Nicotiana tobacum L.by Agrobacterium tumefaciens-mediated genetic transformation,and transgenic plantlets were obtained.The successful integration and expression of the gene was identified by PCR and RT-PCR.The results from determination of salinity resistance indicated that the salinity tolerance of transgenic plants was enhanced. |
| |
| Keywords: | TaNHX2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
