| 细辛组织培养快速繁殖初探 |
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| 引用本文： | 萧洪东,聂磊,蓝旭东,黄锦沛,梁红好.细辛组织培养快速繁殖初探[J].佛山科学技术学院学报(自然科学版),2004,22(2):67-68. |
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| 作者姓名： | 萧洪东 聂磊 蓝旭东 黄锦沛 梁红好 |
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| 作者单位： | 1. 佛山科学技术学院,食品科学系,广东,佛山,528000
2. 斗门区农业技术推广总站,广东,珠海,519000 |
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| 基金项目： | 佛山科技发展专项基金(N0.0102009A) |
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| 摘 要： | 以细辛的根状茎为外植体进行组织培养及继代培养、适合愈伤组织诱导并增殖的培养基为MS+6-BA3.0 mg·L~(-1)+NAA mg·L~(-1);适合诱导再生植株并快速增殖的培养基为MS+6-BA0.8mg·L~(-1)+NAA0.1mg·L~(-1);生根培养基为MS+NAAO.2 mg·L~(-1)+0.5％活性炭。经生根壮苗培养30d左右,移栽后成活率可达98％以上。
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| 关 键 词： | 细辛 组织培养 快速繁殖 |
| 文章编号： | 1008-0171(2004)02-0067-02 |
| 修稿时间： | 2003年5月26日 |
Tissue culture and rapid propagation of Asarum Siboldii Miq. |
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| XIAO Hong-dong,NIE Lei,LAN Xu-dong,HUANG Jin-pei,LIANG Hong-hao.Tissue culture and rapid propagation of Asarum Siboldii Miq.[J].Journal of Foshan University(Natural Science Edition),2004,22(2):67-68. |
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| Authors: | XIAO Hong-dong NIE Lei LAN Xu-dong HUANG Jin-pei LIANG Hong-hao |
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| Institution: | XIAO Hong-dong,,NIE Lei,,LAN Xu-dong,,HUANG Jin-pei,,LIANG Hong-hao Department of Food Science,Foshan Universty,Foshan 528000,China The Center for Spreading Agricultural Technology in Doumen District,Zhuhai 519100,China |
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| Abstract: | The phizome of Asarum Slboldii Mig was used as tissue culture explant. The optimal media
were: (1) MS+6-BA3mg. L~(-1)+NAA0. 1mg. L~(-1) for callus induction and subculture; (2) MS+6-
BA0. 8mg. L~(_1)+NAA0. 1mg. L~(-1) for adventitious bud induction and rapid propagation; (3) MS+
NAA2mg. L~(-1) and 0. 5% active charcoal for shoot rooting. The shoot was planted to the seedbed after
rooting culture for 30 days. The survival rate of transplanting was about 98%. |
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| Keywords: | Asarum siboldii Miq tissue culture rapid propagation |
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