| 猪小肠抗菌肽的提取及部分生物学活性研究 |
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| 引用本文： | 马卫明,佘锐萍,彭芳珍,靳红,胡艳欣.猪小肠抗菌肽的提取及部分生物学活性研究[J].科学技术与工程,2004,4(3):202-205. |
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| 作者姓名： | 马卫明 佘锐萍 彭芳珍 靳红 胡艳欣 |
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| 作者单位： | 中国农业大学动物医学院,北京,100094 |
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| 基金项目： | 国家自然科学基金 ( 3 9870 5 84,3 0 2 70 995 )，高等学校博士学科点专项科研基金 ( 2 0 0 2 0 0 190 2 3 )资助 |
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| 摘 要： | 将新鲜猪小肠超声破碎，经高温处理、乙酸浸提后，再经Sephadex G100和Sephadex G25凝胶柱过滤层析，收集各组分并用琼脂糖弥散法检测各组分的抗菌活性。收集具有抗菌活性的组分，经Tricine SDS-PAGE，考马斯亮蓝染色后分析为一条蛋白带，分子量约为5972Da左右，得到电泳纯的猪小肠抗菌肽。该抗菌肽对大肠杆菌C84008株、金黄色葡萄球菌ATCC25923株、绿脓杆菌ATCC27853株均表现出较强的抗菌活性；并能灭活鸡传染性支气管炎病毒。
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| 关 键 词： | 抗菌肽 猪 小肠 生物学活性 肠道粘膜 抗菌机制 免疫学 |
| 修稿时间： | 2003年11月10 |
The Preparation and Patial Characterization of an Antimicrobial Peptide from the Small Intestine of Pig |
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| MA Weiming,SHE Ruiping,PENG Fangzhen,JIN Hong,HU Yanxin.The Preparation and Patial Characterization of an Antimicrobial Peptide from the Small Intestine of Pig[J].Science Technology and Engineering,2004,4(3):202-205. |
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| Authors: | MA Weiming SHE Ruiping PENG Fangzhen JIN Hong HU Yanxin |
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| Abstract: | After the small intestines of pig were treated by ultrasonic treatment, heating and 5% acetic acid extraction, antimicrobial peptides were isolated from the intestine of pig by Sephadex G100 and Sephadex G25 gel filtration chromatography. The samples were analyzed by Tricine SDS polyacrylamide gel electrophoresis. The results showed that an antimicrobial peptide was purified and its molecule weight was about 5 972 Da. The antimicrobial peptide, named PSIP, strongly inhibited Escherichia coli ATCC84008, Staphylococcus aureus ATCC25923 and Pseudomonas aeruginosa ATCC27853, and neutralized chicken infectious bronchitis virus H120. |
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| Keywords: | antibacterial peptide pig small intestine |
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