功能化介孔二氧化硅微球的药物输送行为分析

介孔二氧化硅(MSNs)作为一类新型的药物载体在药物缓控释领域的应用方兴未艾。采用模板诱导和自组装方法合成MCM-41型介孔二氧化硅纳米颗粒,并通过聚乙二醇(PEG)的表面修饰,制备出一类具有高亲水性、高孔隙率、高比表面积的MSNs修饰微球。采用氮吸附(BET)、扫描电镜(SEM)、透射电镜(TEM)、粒径分析等手段表征并对比了聚乙二醇(PEG)修饰前后的MSNs基础理化性能。在此基础上,选用阿霉素作为模型药物,包埋于介孔材料中,对比了PEG修饰前后MSNs中阿霉素的装载量、包封率及释放行为。选用乳腺癌细胞MCF-7为模型癌细胞,选用人血清蛋白(HAS)作为模型蛋白,考察了两种材料对细胞的生物安全性及对蛋白的吸附情况,并将两种材料所对应的载药微球与MCF-7进行共培养,探究两种载药系统对癌细胞的灭杀情况。

Drug delivery behavior of functional mesoporous silica microspheres

As a new type of drug carriers,Mesoporous silica nanoparticles (MSNs) is in the ascendant in the field of drug control and release. In this article, both the template induced and self-assembling method was employed to synthesize the MCM-41 type of mesoporous silica nanoparticles. With the co-polymerization with polyethylene glycol (PEG), MSNs with wormhole pore structure were obtained with high hydrophilic, high porosity and specific surface area. The modification of PEG to the MSNs was characterized by nitrogen adsorption (BET), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and particle size analysis. Besides, the influence of PEG modification to the drug loading content, the encapsulation efficiency and the release behavior of the MSNs were also analyzed with the doxorubicin as a model drug. To compare the biosecurity and protein adsorption of the two kinds of materials, the human breast cancer cell MCF-7 and the human serum albumin (HAS) were selected as the model cancer cells and the model protein, respectively. On the other hand, to investigate the anti-cancer effect of these two drug delivery systems, the materials were co-cultured with MCF-7 cells and determined the quantity of cells by means of Microplate Reader.