He-Ne激光诱变对分葱外部形态及生理的影响

对He-Ne激光诱变分葱种子幼苗外部形态及生理指标影响的研究有助于进一步研究和指导分葱激光诱变育种工作。实验采用He-Ne激光(功率为0.8mW,波长为632.8nm)分别照射分葱种子0、4、16、32、64、96、128和152min。待种子萌发实验后,移入放有腐质土的育苗盘育苗,45d后对分葱幼苗进行外部形态、叶绿素含量、根系活力、可溶性糖含量、可溶性蛋白含量的测定。在He-Ne激光照射128min处理后,分葱的根长、株高和鲜重等外部形态指标,以及叶绿素a/b和可溶性蛋白含量均表现出一定的促进作用;在96min照射处理后分葱根系活力和可溶性总糖含量也高于其余各组。相反,在4min剂量处理后,发芽率、发芽势、根长、株高和鲜重等外部形态指标,根系活力及叶绿素a/b、可溶性总糖和可溶性蛋白含量等各项指标均低于对照组。总的来看,与较低剂量相比,较高剂量的激光诱变对分葱的生长与新陈代谢活动具有积极的作用。

He-Ne Laser Mutation Effects on External Morphology and Physiology of Allium cepa var. aggregatum

The study about He-Ne laser mutation effects on external morphology and physiology of Allium cepa var. aggregatum, which is named shallot usually, is benefit for the analysis and direction work of laser mutation breeding. In this study, shallot seeds were irradiated by He-Ne laser (0.8 mW, 632.8 nm) for 4, 16, 32, 64, 96, 128 or 152 min respectively. After seeds germination, the shallot seedlings were moved to a seedling tray with humus. And the morphology, chlorophyll content, root activity and soluble sugar content, soluble protein content of shallot seedling were determined 45 days later. The results showed that the root length, plant height, fresh weight, chlorophyll a/b and soluble protein content are promoted by 128 min radiation treating to a certain extent. While the root activity and soluble sugar content of the group treated by 96 min radiation are higher than other groups. Conversely, not only the external morphology such as germination rate, germination energy, root length, plant height and fresh weight, but also root activity, chlorophyll a/b, soluble protein and soluble sugar content of 4 min radiation treated group are all lower than control group. In conclusion, compare to the groups with lower doses, it is suggested that the effect of laser mutation with higher doses is positive on the growth and metabolism of shallot relatively.