| 落叶松种子同工酶的研究 |
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| 引用本文： | 杨茜,乔辰,徐平,张颖娟.落叶松种子同工酶的研究[J].科学技术与工程,2005,5(12):797-799805. |
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| 作者姓名： | 杨茜 乔辰 徐平 张颖娟 |
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| 作者单位： | 1. 内蒙古医学院基础部生物教研室,呼和浩特,010059
2. 内蒙古农业大学农学院,呼和浩特,010019 |
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| 基金项目： | 内蒙古“九五”攻关项目(960108)资助 |
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| 摘 要： | 采用聚丙酰胺凝胶电泳法分析了产地为阿尔山和根河的兴安落叶松L1和L2、华北落叶松L3和长白落叶松L4种子中的4种酶的同工酶。结果表明:4个样品的POD、EST、SOD和AMY同工酶均为多态;同工酶带数目多少为EST>POD>SOD>AMY;同工酶带数目排列是L2>L4>L1>L3。在进化程度上,华北落叶松较为原始,长白落叶松居中,产于根河的兴安落叶松为较进化类型。聚类分析表明长白落叶松与华北落叶松亲缘关系较近,与兴安落叶松较远;华北落叶松与兴安落叶松和长白落叶松亲缘关系均较近。
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| 关 键 词： | 落叶松属 种子 同工酶 聚类分析 相似系数 |
A Study on Isoenzymes in the Seeds of Larix |
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| ANG Qian,QIAO Chen,XU Ping,ZHANG Yingjuan.A Study on Isoenzymes in the Seeds of Larix[J].Science Technology and Engineering,2005,5(12):797-799805. |
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| Authors: | ANG Qian QIAO Chen XU Ping ZHANG Yingjuan |
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| Abstract: | Four isoenzymes among the seeds of Larix gmelini(Rupr.) from Aershan(L1) and Genhe(L2), L. principis-rupprechtii Mayr.(L3)and L. olgensis Henry(L4)were analysed by means of polyacrylamide gel electrophoresis. The results showed that the isoenzymes of POD, EST, SOD and AMY in four samples wer all polymorphic. The mumber of the isoenzymes bands showed following tendency:L2>L4>L1>L3 . To extent of evolution, L. principis-rupprechtii is more original, L. olgensis is medial and L. gmelini from Genhe belonged to the type of evolution. The result of cluster analysis showed that L. olgensis had a closer relationship with L. principis-rupprechtii, and had a further relationship with L. gmelini. L. principis-rupprechtii had relatively close relationship with L. gmelini and L. olgensis. |
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| Keywords: | Larix Mill seed isoenzyme cluster analysis proximity coefficient |
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