基于免泵微流控芯片和电化学检测的便携式生物医学传感器研究

为满足即时检验(point-of-care testing,POCT)在基层和个性化诊疗使用需求,开发了一种免泵式微流控芯片和电化学检测生物医学传感器系统,配合免疫传感器完成了对过氧化氢和前列腺癌标志物的快速检测。系统利用改性处理后聚二甲基硅氧烷芯片通道内毛细作用力,自泵式完成待测试剂混合和流动,采用微型便携式检测设备控制输入信号并通过微电流检测电路设计读取传感器上电流信号,实现快速自动化检测。实验验证了三电极检测电路输出电位稳定,误差不超过1%;通过对双氧水浓度检测实验证明了该检测体系对浓度变化电流动态响应特性良好,输出稳定;检测系统配合生物传感器对前列腺特异性抗原浓度进行定量检测,检测限为1 ng/m L。据我们所知,这是首次利用电化学免泵微流控传感器进行前列腺抗原的检测,本体系结构简单,操作简便,系统有望与多种电化学传感器结合用于多种检测物质的快速智能化POCT检测。

Development of A Portable Biomedical Sensor Based on Pump-free Microfluidic Chip and Electrochemical Detection

In order to satisfy requirement of point-of-care test(POCT) for rapid detection at primary-level clinics and personal users, a novel amperometric electrochemistry sensor detection system was developed and combined with pump-free microfluidic chip used for H2O2 and prostate cancer markers detection. Capillary force was utilized to modify polydimethylsiloxane channel for adding and mixing samples, a portable three-electrode detector was designed to control input signals and receive current signal on electrode sensor. This system was certificated by the portable detection circuit output voltage stability, and the error accuracy not exceed 1%. Meanwhile, detection of hydrogen peroxide showed good current dynamic response of concentration change. Prostate specific antigen was quantitatively detected with a limit of detection of 1ng/mL. To the best of our knowledge, this is the first time that PSA was detected using electrochemistry and pump-free microfluidic chip. It is a simple portable electrochemistry detection system based on pump-free microfluidic chip and expect to combine with different electrochemical sensors for rapid intelligent POCT detection.