响应面优化假单胞杆菌降解咖啡因培养基

利用已筛选出的菌株Pseudomonas putida ECUST5-2降解咖啡因,并对其培养基组分进行优化。首先采用单因子试验从多个因素中寻找出可参考的因素。在此基础之上,利用Plackett-Burman试验筛选出显著影响咖啡因降解率的主要因素为:咖啡因、Na2HPO4、MgSO4和酵母粉。随后用最陡爬坡试验逼近降解菌最大降解率区域。然后利用中心组合设计试验对相关显著因素进行优化,得到咖啡因、Na2HPO4、MgSO4和酵母粉的最佳浓度分别为:3.409 1 g·L-1,0.056 4 g·L-1,0.1611g·L-1和3.570 7 g·L-1。在优化后培养基条件下,咖啡因的降解率(0.105 g·h-1)比其初始咖啡因降解率提高了3.28倍。

Optimization of degradation medium for caffeine with response surface method

Degradation of caffeine by screened Pseudomonas putida ECUST5-2 and optimization of culture medium is studied. Reference ranges of each factor are firstly determined with single factorial experiment and four major factors significantly affected degradation of caffeine, which are caffeine, Na2HPO4, MgSO4 and yeast extracts are determined by Plackett-Burman design. The optimum fermentation conditions are optimized with Central composite design experiments and the largest regional of caffeine degradation rate is approach with the steepest ascent experiment. The optimum composition of medium is addition of caffeine, Na2HPO4, MgSO4 and yeast extracts is 3.4091 g L-1, 0.0564 g L-1, 0.1611 g L-1 and 3.5707 g L-1