参藤三黄汤对糖尿病肾病中MMP2,9及TIMP1,2 基因和蛋白表达的影响

探索参藤三黄汤对糖尿病肾病中MMP2,9、TIMP1,2基因和蛋白表达的影响,以50只实验大鼠随即分为空白组10只,余下造模成功后等分为模型组,参藤三黄汤高、低剂量组,阳性药对照组并给予相应药物。12周后实验结束时检测大鼠24 h尿微量白蛋白、血糖、血胰岛素等指标及肾内皮细胞外基质MMP2,9、TIMP1,2基因和蛋白的表达。结果:1参藤三黄汤治疗组血糖水平明显优于模型组,差异显著(P0.01),说明参藤三黄汤有明显的降血糖功效;2参藤三黄汤干预后,治疗组大鼠的糖耐量异常有所改善,与模型组比较,差异有统计学意义(P0.05);治疗组大鼠肾小球和肾小管形态趋于正常且MMP2,9表达明显提高,而TIMP1,2表达明显降低。说明参藤三黄汤能明显降低糖尿病大鼠的血糖水平,增强糖耐量能力;其作用机制可能与提高MMP2,9、降低TIMP1,2基因和蛋白的表达有关。

Shen teng san huang liquid On diabetic nephropathy in MMP2,9 and TIMP1,2 gene and protein expression

SObjective:To exploreStheSShen teng san huangSliquid onSdiabetic nephropathySinSMMP2,S9,STIMP1,S2Sgene and protein expression.SMethods: 50Srats wereSrandomly dividedSinto blank groupS10,Sthe remainingSafter the success of modelingSis divided intoSmodel group,SShen teng san huangSliquidShigh,Slow dose group,Spositive drug control groupSandSgiven corresponding drugs.SThe end of the experimentS12 weeks afterSrat were examined atS24 hSurinary albumin,Sblood glucose,SinsulinSand other indicatorsSand renalSendothelial cell extracellular matrixSMMP2,S9,STIMP1,expression of 2Sgenes and proteins.SResults: 1SShen teng san huangSliquid blood glucose levelSwas significantly better than the model group,Ssignificant differenceS(P lt;S0.01),StheSShen teng san huangSliquidShasSobviousSeffect of lowering blood sugar.S2SShen teng san huangSliquidSintervention,Sthe amount ofSabnormalSimprovedSglucose toleranceSof the rats in the treatment group,Scompared with model group,the difference was statistically significantS(P lt;S0.05);Streatment groupSratSglomerular and tubularSmorphologytended to be normalSandSMMP2S9Sexpression,Sincreased significantly,Swhile TIMP1Sexpression was significantly decreased,S2.SConclusion:SShen teng san huangSliquidScan significantly reduce theSblood glucose level in diabetic rats,Senhance theScapacity ofSglucose tolerance;Sthe mechanismSmay be related to the increase ofSMMP2,decreaseS9,STIMP1,Sexpression ofS2 gene and protein.